柚皮素诱导口腔癌细胞凋亡的 ROS 介导的 Bid 和 Bcl-xl 信号通路。
Naringenin-induced Oral Cancer Cell Apoptosis ROS-mediated Bid and Bcl-xl Signaling Pathway.
机构信息
Department of Kashan Outpatient Clinic, Affiliated Stomatology Hospital of Guangzhou Medical University, Guangdong Engineering Research Center of Oral Restoration and Reconstruction, Guangzhou Key Laboratory of Basic and Applied Research of Oral Regenerative Medicine, Guangzhou, Guangdong, 510182, Chin.
Center Laboratory, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, No 318 Renminzhong Road, Yuexiu District, Guangzhou, 510120, China.
出版信息
Curr Cancer Drug Targets. 2024;24(6):668-679. doi: 10.2174/0115680096267430231023091521.
BACKGROUND
Oral cancer is a malignant tumor with a high impact and poor prognosis. Naringenin, a flavonoid found in citrus fruits and its anti-inflammatory and antioxidant properties offer potential therapeutic benefits. However, limited studies have been conducted on the impact of naringenin on human tongue carcinoma CAL-27 cells. This study aims to elucidate the correlation between naringenin and tongue cancer, thereby identifying a potential therapeutic candidate for drug intervention against tongue cancer.
METHODS
The effect of naringenin on the apoptosis of CAL-27 cells and its mechanism were studied by cell counting kit-8, mitochondrial membrane potential assay with JC-1, Annexin V-- FITC apoptosis detection, cell cycle, and apoptosis analysis, Reactive Oxygen Species assay and Western blot.
RESULTS
The results showed that naringenin significantly induced apoptosis in CAL-27 cells in a dose-dependent manner. Mechanistically, naringenin-induced apoptosis was mediated through the upregulation of Bid and downregulation of Bcl-xl, which led to increased generation of ROS.
CONCLUSION
The findings suggested that naringenin may represent a promising candidate for the treatment of oral cancer by inducing apoptotic cell death via modulation of the Bid and Bcl-xl signaling pathways.
背景
口腔癌是一种恶性肿瘤，具有高影响力和预后不良。柚皮素是一种在柑橘类水果中发现的类黄酮，具有抗炎和抗氧化特性，提供了潜在的治疗益处。然而，关于柚皮素对人舌癌细胞 CAL-27 的影响的研究有限。本研究旨在阐明柚皮素与舌癌之间的相关性，从而确定一种潜在的治疗候选物，用于药物干预舌癌。
方法
通过细胞计数试剂盒-8、JC-1 线粒体膜电位测定法、Annexin V-FITC 凋亡检测、细胞周期和凋亡分析、活性氧（ROS）测定和 Western blot 研究了柚皮素对 CAL-27 细胞凋亡的影响及其机制。
结果
结果表明，柚皮素以剂量依赖的方式显著诱导 CAL-27 细胞凋亡。在机制上，柚皮素诱导的细胞凋亡是通过上调 Bid 和下调 Bcl-xl 介导的，这导致 ROS 的产生增加。
结论
研究结果表明，柚皮素可能通过调节 Bid 和 Bcl-xl 信号通路诱导细胞凋亡死亡，成为治疗口腔癌的有前途的候选药物。
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