School of Food and Health, Beijing Technology and Business University, Beijing 100048, PR China; Food Safety Key Laboratory of Zhejiang Province, School of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou 310018, PR China.
Food Safety Key Laboratory of Zhejiang Province, School of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou 310018, PR China.
Int J Food Microbiol. 2024 Feb 16;412:110553. doi: 10.1016/j.ijfoodmicro.2023.110553. Epub 2024 Jan 2.
Pseudomonas fluorescens is a spoilage bacterium in food that has the ability to maintain growth and reproduction in high-salt environments. It acts as a defence mechanism through the exclusion of ions and the formation of biofilms. Hence, disrupting this defence mechanism may be a good way to control food spoilage. In this study, a specific flavonoid small molecule baicalin was found, which was able to dismantle the defence mechanism of the bacteria at a lower concentration (400 μM) of treatment. In synergy with salt, baicalin showed a significant inhibitory effect on the growth, c-di-gmp synthetics and biofilm formation of Pseudomonas fluorescens Pf08. Through transcriptomics, we also found that baicalein interfered with bacterial transport and polysaccharide production functions. Through molecular docking and QPCR, we found that baicalin is able to binding with the RpoS protein through hydrogen bonding and thus interfere with its function.
荧光假单胞菌是一种食品腐败菌，能够在高盐环境中维持生长和繁殖。它通过排除离子和形成生物膜来充当防御机制。因此，破坏这种防御机制可能是控制食物腐败的一种好方法。在这项研究中，发现了一种特定的黄酮类小分子黄芩苷，它能够在较低浓度（400μM）的处理下瓦解细菌的防御机制。黄芩苷与盐协同作用，对荧光假单胞菌 Pf08 的生长、c-di-GMP 合成和生物膜形成有显著的抑制作用。通过转录组学，我们还发现黄芩苷干扰了细菌的运输和多糖产生功能。通过分子对接和 QPCR，我们发现黄芩苷能够通过氢键与 RpoS 蛋白结合，从而干扰其功能。
