Phan Quoc-Hung, Dinh Quoc-Thinh, Pan Yi-Cheng, Huang Yi-Ting, Hong Zi-Hao, Lu Tzu-Shiang
Mechanical Engineering Department, National United University, Miaoli 36063, Taiwan.
Mechanical Engineering Department, National United University, Miaoli 36063, Taiwan.
Talanta. 2024 Apr 1;270:125611. doi: 10.1016/j.talanta.2023.125611. Epub 2024 Jan 1.
A decomposition Mueller matrix method is proposed for detection of miRNA and enhanced by using a surface plasmon resonance (SPR). In the proposed approach, a Mueller matrix decomposition method is employed to extract the linear birefringence (LB) and circular dichroism (CD) properties of the miRNA sample. The accuracy of the LB and CD measurements is enhanced through the use of a high-resolution antimonene-based SPR prism coupler with DNA-linked gold nanoparticles (AuNPs). The feasibility of the proposed method is demonstrated by measuring the LB orientation angle (α) and CD property (R) of two miRNA aqueous solutions (hsa-miR-125-5p and hsa-miR-21-5p) over the concentration range of 0∼1000 fM in both cases. The results show that, for both samples, α and R vary linearly with the change in the miRNA concentration. Furthermore, the values of α and R obtained for the two samples are quantifiably different, and hence the selectivity of the proposed SPR sensor is confirmed. Overall, the results highlight the potential of the proposed sensor as a valuable tool for miRNA detection with prospective applications in cancer diagnosis.
提出了一种用于检测微小RNA（miRNA）的分解穆勒矩阵方法，并通过使用表面等离子体共振（SPR）进行了增强。在所提出的方法中，采用穆勒矩阵分解方法来提取miRNA样品的线性双折射（LB）和圆二色性（CD）特性。通过使用带有DNA连接金纳米颗粒（AuNP）的高分辨率锑烯基SPR棱镜耦合器，提高了LB和CD测量的准确性。通过测量两种miRNA水溶液（hsa-miR-125-5p和hsa-miR-21-5p）在0至1000 fM浓度范围内的LB取向角（α）和CD特性（R），证明了该方法的可行性。结果表明，对于两个样品，α和R均随miRNA浓度的变化呈线性变化。此外，两个样品获得的α和R值在数量上是不同的，因此证实了所提出的SPR传感器的选择性。总体而言，结果突出了所提出的传感器作为一种用于miRNA检测的有价值工具的潜力，在癌症诊断中具有潜在应用。
