基于黑磷烯的表面等离子体共振传感器超灵敏检测 miRNA。

Ultrasensitive detection of miRNA with an antimonene-based surface plasmon resonance sensor.

机构信息

Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Electronic Science and Technology and College of Optoelectronics Engineering, Shenzhen University, 518060, Shenzhen, People's Republic of China.

School of Materials Science and Engineering and Key Laboratory of Automobile Materials of MOE, Jilin University, 130012, Changchun, Jilin, People's Republic of China.

出版信息

Nat Commun. 2019 Jan 3;10(1):28. doi: 10.1038/s41467-018-07947-8.

MicroRNA exhibits differential expression levels in cancer and can affect cellular transformation, carcinogenesis and metastasis. Although fluorescence techniques using dye molecule labels have been studied, label-free molecular-level quantification of miRNA is extremely challenging. We developed a surface plasmon resonance sensor based on two-dimensional nanomaterial of antimonene for the specific label-free detection of clinically relevant biomarkers such as miRNA-21 and miRNA-155. First-principles energetic calculations reveal that antimonene has substantially stronger interaction with ssDNA than the graphene that has been previously used in DNA molecule sensing, due to thanking for more delocalized 5s/5p orbitals in antimonene. The detection limit can reach 10 aM, which is 2.3-10,000 times higher than those of existing miRNA sensors. The combination of not-attempted-before exotic sensing material and SPR architecture represents an approach to unlocking the ultrasensitive detection of miRNA and DNA and provides a promising avenue for the early diagnosis, staging, and monitoring of cancer.

微 RNA 在癌症中表现出不同的表达水平，可影响细胞转化、癌变和转移。虽然已经研究了使用染料分子标记的荧光技术，但 miRNA 的无标记分子水平定量极其具有挑战性。我们开发了一种基于二维纳米材料锑烯的表面等离子体共振传感器，用于特异性无标记检测临床相关生物标志物，如 miRNA-21 和 miRNA-155。第一性原理能量计算表明，由于锑烯中的 5s/5p 轨道更具离域性，因此与先前用于 DNA 分子传感的石墨烯相比，锑烯与 ssDNA 的相互作用要强得多。检测限可达到 10 aM，比现有 miRNA 传感器的检测限高 2.3-10,000 倍。前所未有的奇异传感材料与 SPR 结构的结合为实现 miRNA 和 DNA 的超灵敏检测提供了一种方法，并为癌症的早期诊断、分期和监测提供了有前景的途径。