Sumi Y, Nakamura Y, Aoki N, Sakai M, Muramatsu M
J Biochem. 1986 Nov;100(5):1399-402. doi: 10.1093/oxfordjournals.jbchem.a121846.
A 1.7 kilobase cDNA clone isolated from a human liver cDNA library contained 822 nucleotides encoding the carboxyl-terminal 274 amino acid sequence of alpha 2-plasmin inhibitor, a stop codon and a 3' noncoding region of 0.9 kilobases. The amino acid sequence deduced from the cDNA shows 29-31% homology with those of other plasma protease inhibitors. The inhibitor's putative reactive-site peptide bond was Met-Ser, and the Met residue was located at the 91st position from the carboxyl-terminal end. The plasminogen binding site was located in the carboxyl-terminal region.
从人肝脏cDNA文库中分离出的一个1.7千碱基的cDNA克隆，包含822个核苷酸，编码α2 - 纤溶酶抑制剂的羧基末端274个氨基酸序列、一个终止密码子以及一个0.9千碱基的3'非编码区。从该cDNA推导的氨基酸序列与其他血浆蛋白酶抑制剂的氨基酸序列显示出29 - 31%的同源性。该抑制剂假定的活性位点肽键为甲硫氨酸 - 丝氨酸，甲硫氨酸残基位于从羧基末端起的第91位。纤溶酶原结合位点位于羧基末端区域。
