Cai Li, Hou Baojian, Hu Juping
Department of Endocrinology, the Third People's Hospital of Hubei Province, Wuhan, Hubei, China.
Arch Physiol Biochem. 2024 Dec;130(6):848-853. doi: 10.1080/13813455.2023.2298404. Epub 2024 Jan 8.
To investigate the effects of tomatidine (Td) on the progression of type 2 diabetes mellitus (T2DM) in mice and uncover the mechanism.
T2DM mice model was induced by high-fat diet (HFD) and intrabitoneal injection of streptozotocin (STZ). The mice were grouped as follows: 1, control; 2, T2D; 3, T2D + tomatidine (5 mg/kg); 4, T2D + tomatidine (10 mg/kg); 5, T2D + tomatidine (20 mg/kg). Fasting blood glucose was detected by glucose metre and fasting insulin was detected by the kit to determine the effect of Td on T2DM mice. ELISA, qPCR, and Immunoblot assays were performed to detect the effects of Td on the hepatic glucose homeostasis and inflammation of mice. Immunoblot assays further confirmed the mechanism.
Td improved blood glucose and insulin resistance in T2DM mice. In addition, Td improved liver function and lipid metabolism disorder in T2DM mice. Td also affected the liver glucose homeostasis related genes in T2DM mice. Td alleviated serum inflammation in T2DM mice. We further found that Td activated AMPK pathway, therefore ameliorating T2DM.
Td ameliorated HFD/STZ-induced T2DM in mice, suggesting that it could serve as a drug of T2DM.
研究番茄碱（Td）对小鼠2型糖尿病（T2DM）进展的影响并揭示其机制。
通过高脂饮食（HFD）和腹腔注射链脲佐菌素（STZ）诱导建立T2DM小鼠模型。将小鼠分为以下几组：1，对照组；2，T2D组；3，T2D + 番茄碱（5 mg/kg）组；4，T2D + 番茄碱（10 mg/kg）组；5，T2D + 番茄碱（20 mg/kg）组。用血糖仪检测空腹血糖，用试剂盒检测空腹胰岛素，以确定Td对T2DM小鼠的作用。进行酶联免疫吸附测定（ELISA）、定量聚合酶链反应（qPCR）和免疫印迹分析，以检测Td对小鼠肝脏葡萄糖稳态和炎症的影响。免疫印迹分析进一步证实其机制。
Td改善了T2DM小鼠的血糖和胰岛素抵抗。此外，Td改善了T2DM小鼠的肝功能和脂质代谢紊乱。Td还影响了T2DM小鼠肝脏中与葡萄糖稳态相关的基因。Td减轻了T2DM小鼠的血清炎症。我们进一步发现Td激活了AMPK通路，从而改善了T2DM。
Td改善了HFD/STZ诱导的小鼠T2DM，表明它可作为一种治疗T2DM的药物。
