Navarro J, Vidal F, Templado C, Benet J, Marina S, Pomerol J M, Egozcue J
Hum Reprod. 1986 Dec;1(8):523-7. doi: 10.1093/oxfordjournals.humrep.a136467.
Mitotic and meiotic chromosome studies and synaptonemal complex analyses by light and electron microscopy have been carried out in a selected series of 47 infertile or sterile males with highly abnormal seminograms, affecting the number of spermatozoa, their morphology and/or motility. In 46 cases, the karyotype was 46,XY. One patient had a 13/14 translocation. With the exception of the patient with a 13/14 translocation, and three patients with desynapsis (8.5%), all other cases showed either normal or absent metaphase I figures. However, synaptonemal complex analysis by light and electron microscopy demonstrated the presence of pairing anomalies (desynapsis, fragmented or irregular synaptonemal complexes) in 31.9% of the patients studied. The total number of synaptic anomalies observed (40.4%) is higher than in a former light microscopy study of 111 infertile or sterile patients (28.8%) probably because the higher resolution of the electron microscope permits the characterization of some anomalies that cannot be detected with the light microscope. The electron microscope should therefore be used in all cases in which the light microscope provides doubtful results.
我们对47名不育或无精男性进行了有丝分裂和减数分裂染色体研究，并通过光学显微镜和电子显微镜对联会复合体进行了分析。这些男性的精液分析结果高度异常，精子数量、形态和/或活力均受影响。46例患者的核型为46,XY。1例患者存在13/14易位。除1例13/14易位患者和3例联会消失患者（8.5%）外，所有其他病例的中期I图像均正常或缺失。然而，通过光学显微镜和电子显微镜对联会复合体进行分析发现，在31.9%的研究患者中存在配对异常（联会消失、联会复合体片段化或不规则）。观察到的突触异常总数（40.4%）高于之前对111名不育或无精患者进行的光学显微镜研究（28.8%），这可能是因为电子显微镜的分辨率更高，能够识别一些光学显微镜无法检测到的异常。因此，在光学显微镜检查结果存疑的所有病例中均应使用电子显微镜。
