Department of Molecular and Chemical Life Sciences, Graduate School of Life Sciences, Tohoku University, Sendai, Japan.
Biosci Biotechnol Biochem. 2024 Feb 21;88(3):305-315. doi: 10.1093/bbb/zbad178.
Acidovorax sp. KKS102 is a beta-proteobacterium capable of degrading polychlorinated biphenyls (PCBs). In this study, we examined its growth in liquid nutrient broth supplemented with different carbon sources. KKS102 had at least 3 distinct metabolic phases designated as metabolic phases 1-3, with phase 2 having 2 sub-phases. For example, succinate, fumarate, and glutamate, known to repress the PCB/biphenyl catabolic operon in KKS102, were utilized in phase 1, while acetate, arabinose, and glycerol in phase 2, and glucose and mannose in phase 3. We also showed that the BphQ response regulator mediating catabolite control in KKS102, whose expression level increased moderately through the growth, plays important roles in carbon metabolism in phases 2 and 3. Our study elucidates the hierarchical growth of KKS102 in nutrient-rich media. This insight is crucial for studies exploiting microbial biodegradation capabilities and advancing studies for catabolite regulation mechanisms.
酸噬草酸杆菌（Acidovorax sp.）KKS102 是一种能够降解多氯联苯（PCBs）的β变形菌。在本研究中，我们研究了其在补充不同碳源的液体营养肉汤中的生长情况。KKS102 至少有 3 个不同的代谢阶段，分别命名为代谢阶段 1-3，其中阶段 2 有 2 个子阶段。例如，琥珀酸、富马酸和谷氨酸，已知在 KKS102 中抑制 PCB/联苯分解操纵子，在阶段 1 被利用，而乙酸盐、阿拉伯糖和甘油在阶段 2，葡萄糖和甘露糖在阶段 3。我们还表明，BphQ 响应调节剂介导 KKS102 中的分解代谢物控制，其表达水平在生长过程中适度增加，在阶段 2 和 3 的碳代谢中发挥重要作用。我们的研究阐明了 KKS102 在营养丰富的培养基中的分级生长。这一见解对于利用微生物生物降解能力的研究和推进分解代谢物调节机制的研究至关重要。
