Guangdong Provincial Key Laboratory of Nutraceuticals and Functional Foods, College of Food Sciences, South China Agricultural University, Guangzhou, China.
Department of Food Science and Nutrition, College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou, China.
Food Funct. 2024 Feb 5;15(3):1265-1278. doi: 10.1039/d3fo04378a.
Nobiletin (NOB) exhibits significant biological activities and may be a potential dietary treatment for antibiotic-associated gut dysbiosis. In this study, mice were gavaged with 0.2 mL day of 12.5 g L cefuroxime (LFX) and 10 g L levofloxacin (LVX) for a duration of 10 days, accompanied by 0.05% NOB to investigate the regulatory effect and potential mechanisms of NOB on antibiotic-induced intestinal microbiota disorder and intestinal barrier dysfunction. Our results indicated that dietary NOB improved the pathology of intestinal epithelial cells and the intestinal permeability by upregulating the expression of intestinal tight junction proteins (TJs) and the number of goblet cells. Furthermore, dietary NOB reduced the levels of serum lipopolysaccharide (LPS) and pro-inflammatory factors (TNF-α and IL-1β), thereby facilitating the restoration of the intestinal mucosal barrier. Additionally, dietary NOB increased the abundance of beneficial bacteria and regulated the metabolic disorders of short-chain fatty acids (SCFAs) and bile acids (BAs). Notably, NOB supplementation resulted in elevated levels of butyric acid and lithocholic acid (LCA), which contributed to the repair of the intestinal mucosal barrier function and the maintenance of intestinal homeostasis. Collectively, our results propose a healthy dietary strategy for the prevention or mitigation of antibiotic-associated gut dysbiosis by dietary NOB.
川陈皮素（NOB）具有显著的生物学活性，可能是治疗抗生素相关性肠道菌群失调的潜在饮食疗法。在这项研究中，通过灌胃给予小鼠 12.5 g/L 头孢呋辛（LFX）和 10 g/L 左氧氟沙星（LVX），每天 0.2 mL，持续 10 天，并添加 0.05%的 NOB，以研究 NOB 对抗生素诱导的肠道微生物群失调和肠道屏障功能障碍的调节作用及潜在机制。结果表明，饮食 NOB 通过上调肠道紧密连接蛋白（TJs）和杯状细胞数量，改善了肠道上皮细胞病理和肠道通透性。此外，饮食 NOB 降低了血清脂多糖（LPS）和促炎因子（TNF-α和 IL-1β）水平，从而促进了肠道黏膜屏障的恢复。此外，饮食 NOB 增加了有益细菌的丰度，并调节了短链脂肪酸（SCFAs）和胆汁酸（BAs）的代谢紊乱。值得注意的是，NOB 补充剂可增加丁酸和石胆酸（LCA）的水平，有助于修复肠道黏膜屏障功能和维持肠道内环境稳态。总之，我们的研究结果提出了一种通过饮食 NOB 预防或减轻抗生素相关性肠道菌群失调的健康饮食策略。
