Jilin Provincial Key Laboratory of Tree and Grass Genetics and Breeding, College of Forestry and Grassland Science, Jilin Agricultural University, Changchun, China.
National Key Laboratory of Forest Genetics and Breeding, Northeast Forestry University, Harbin, China.
Microbiol Spectr. 2024 Feb 6;12(2):e0336823. doi: 10.1128/spectrum.03368-23. Epub 2024 Jan 10.
Terrestrial plants can influence the growth and health of adjacent plants through interspecific interaction. Here, the mechanisms of interspecific plant interaction on microbial function and nutrient utilization in the plant-soil interface (non-rhizosphere soil, rhizosphere soil, and root) were studied by soybean- and potato-poplar intercropping. First, metagenomics showed that soybean- and potato-poplar intercropping influenced the composition and co-occurrence networks of microbial communities in different ecological niches, with higher stability of the microbial community in soybean intercropping. Second, the gene abundance related to carbon metabolism, nitrogen cycling, phosphorus cycling, and sulfur cycling was increased at the poplar-soil interface in soybean intercropping. Moreover, soybean intercropping increased soil nutrient content and enzymatic activity. It showed higher metabolic potential in nutrient metabolism and transportation. Third, functional microorganisms that influenced nutrient cycling and transportation in different intercropping have been identified, namely , , , , and . Therefore, intercropping can construct microbial communities to alter metabolic functions and improve nutrient cycling and absorption. Interspecific plant interactions to influence the microbiome were revealed, opening up a new way for the precise regulation of plant microbiome.IMPORTANCEPoplar has the characteristics of wide distribution, strong adaptability, and fast growth, which is an ideal tree species for timber forest. In this study, metagenomics and elemental analysis were used to comprehensively reveal the effects of interspecific plant interactions on microbial communities and functions in different ecological niches. It can provide a theoretical basis for the development and application of the precise management model in poplar.
陆生植物可以通过种间相互作用影响相邻植物的生长和健康。在这里，通过大豆-马铃薯-杨树间作，研究了种间植物相互作用对植物-土壤界面（非根际土壤、根际土壤和根）中微生物功能和养分利用的影响机制。首先，宏基因组学表明，大豆-马铃薯-杨树间作对不同生态位微生物群落的组成和共生网络产生了影响，大豆间作中微生物群落的稳定性更高。其次，与碳代谢、氮循环、磷循环和硫循环相关的基因丰度在大豆间作的杨树-土壤界面增加。此外，大豆间作增加了土壤养分含量和酶活性。它在养分代谢和运输方面表现出更高的代谢潜力。第三，鉴定出了影响不同间作中养分循环和运输的功能微生物，即、、、、和。因此，间作可以构建微生物群落，改变代谢功能，提高养分循环和吸收。揭示了种间植物相互作用对微生物组的影响，为植物微生物组的精确调控开辟了新途径。
重要的是，杨树分布广泛、适应性强、生长迅速，是理想的用材林树种。在本研究中，宏基因组学和元素分析被用于全面揭示种间植物相互作用对不同生态位微生物群落和功能的影响。它可为杨树精确管理模型的开发和应用提供理论依据。
