Yang Xiaojuan, Deng Biyu, Lu Shiyi, Wang Cong, Liang Yongyan, Liu Shinan
Guangxi Key Laboratory of Forest Ecology and Conservation, College of Forestry, Guangxi University, Nanning, China.
Guangxi Forest Resources and Environment Monitoring Center, Nanning, China.
Front Microbiol. 2025 Mar 27;16:1548836. doi: 10.3389/fmicb.2025.1548836. eCollection 2025.
is an endangered species belonging to the Cupressaceae family. The comprehensive examination of soil characteristics and rhizosphere microbial communities is vital for conservation efforts, as it provides insights into the necessary environmental conditions for safeguarding and ensuring the viability of rare and endangered species. In this study, the diversity and composition of bacterial and fungal communities were compared in the roots and rhizosphere soils of cultivated and wild in Guangxi, China. The results revealed that, at the phylum level, the rhizosphere of cultivated was significantly enriched with , , , and , while wild was significantly enriched with , , and . Symbiotic network analysis indicated that the bacterial network in the cultivated rhizosphere had higher edge values, average degree, clustering coefficient, and network density, while the fungal network in the wild rhizosphere had higher node values, edge values, average degree, and clustering coefficient. Moreover, functional prediction results suggested that bacteria in cultivated showed higher metabolic activity, with fungi primarily acted as saprotrophs and symbionts. In contrast, bacteria in wild displayed lower metabolic activity, with fungi predominantly functioning as saprotrophs. The analysis linking rhizospheric microbial diversity to soil environmental factors showed a closer association for the wild microbial community, suggesting a stronger influence of soil environmental factors. The Random Forest (RF) highlighted that the total phosphorus and total potassium levels were key influencing factors for rhizospheric microbes in cultivated , while available potassium levels were crucial for those in wild . These differences underscore the significant strategies for in adapting to different habitats, which may be intricately linked to land management practices and soil environmental factors. Among these, phosphorus and potassium are significantly associated with the rhizosphere microorganisms of . Therefore, continuous monitoring of nutrient availability and regular supplementation of phosphorus and potassium fertilizers in the rhizosphere are recommended during the cultivation and ex-situ conservation of .
是柏科的一种濒危物种。对土壤特性和根际微生物群落进行全面检查对于保护工作至关重要，因为它能为保护珍稀濒危物种并确保其生存能力所需的环境条件提供见解。在本研究中，对中国广西栽培和野生的根及根际土壤中细菌和真菌群落的多样性与组成进行了比较。结果表明，在门水平上，栽培的根际显著富集了 、 、 和 ，而野生的则显著富集了 、 和 。共生网络分析表明，栽培的根际细菌网络具有更高的边值、平均度、聚类系数和网络密度，而野生的根际真菌网络具有更高的节点值、边值、平均度和聚类系数。此外，功能预测结果表明，栽培的中的细菌表现出更高的代谢活性，真菌主要作为腐生菌和共生菌发挥作用。相比之下，野生的中的细菌代谢活性较低，真菌主要发挥腐生菌的功能。将根际微生物多样性与土壤环境因素联系起来的分析表明，野生的微生物群落关联更紧密，表明土壤环境因素的影响更强。随机森林（RF）突出显示，总磷和总钾水平是栽培的根际微生物的关键影响因素，而有效钾水平对野生的根际微生物至关重要。这些差异凸显了在适应不同栖息地方面的重要策略，这可能与土地管理实践和土壤环境因素密切相关。其中，磷和钾与的根际微生物显著相关。因此，建议在栽培和迁地保护期间持续监测养分有效性，并定期在根际补充磷钾肥。
