Mitsumoto H, Boggs A L
J Neuropathol Exp Neurol. 1987 Mar;46(2):214-22. doi: 10.1097/00005072-198703000-00008.
We investigated whether vacuolated cervical anterior horn cells of the wobbler mouse maintain axons to the periphery, and if these morphologically abnormal neurons are capable of supporting axonal regeneration. Using retrograde axonal transport, we applied horseradish peroxidase (HRP) to peripheral nerves or muscles and with electron microscopy sought evidence for perikaryal labeling in vacuolated neurons in 23 wobbler mice. When HRP was injected into forelimb muscles, 12 of 36 vacuolated neurons became positively labeled indicating that these neurons have axons in continuity with the periphery. In regeneration studies, after nerve crush at the brachial plexus, 23 out of 85 vacuolated neurons were labeled after HRP application at the elbow level. However, after a sufficient regeneration period, none of the 36 vacuolated neurons were labeled if HRP was applied in muscles below the elbow. In all experiments, morphologically normal neurons were always labeled. Our studies indicate that some vacuolated neurons of wobbler mice not only maintain axons into the periphery, but are also capable of supporting regeneration. However, the overall function of these vacuolated neurons appears marginal compared with the majority of morphologically normal neurons in this motor neuron disease.
我们研究了摇摆小鼠颈前角空泡化细胞是否保留了通向周围的轴突，以及这些形态异常的神经元是否能够支持轴突再生。利用逆行轴突运输，我们将辣根过氧化物酶（HRP）应用于周围神经或肌肉，并通过电子显微镜在23只摇摆小鼠的空泡化神经元中寻找核周标记的证据。当将HRP注入前肢肌肉时，36个空泡化神经元中有12个呈阳性标记，表明这些神经元具有与周围相连的轴突。在再生研究中，在臂丛神经挤压伤后，在肘部水平应用HRP后，85个空泡化神经元中有23个被标记。然而，经过足够的再生期后，如果在肘部以下的肌肉中应用HRP，36个空泡化神经元中没有一个被标记。在所有实验中，形态正常的神经元总是被标记。我们的研究表明，摇摆小鼠的一些空泡化神经元不仅保留了通向周围的轴突，而且还能够支持再生。然而，与这种运动神经元疾病中大多数形态正常的神经元相比，这些空泡化神经元的整体功能似乎有限。
