Hubei Key Laboratory of Animal Nutrition and Feed Science, Hubei Collaborative Innovation Center for Animal Nutrition and Feed Safety, Wuhan Polytechnic University, Wuhan, China.
School of Life and Environmental Sciences, Wenzhou University, Wenzhou, China.
J Phycol. 2024 Apr;60(2):432-446. doi: 10.1111/jpy.13426. Epub 2024 Jan 10.
To increase the understanding of simple thin filamentous cyanobacteria in harsh environmental areas, we previously isolated and identified four strains (XN101, XN102, GS121, NX122) from desert soils and hot spring in China. As a result, two new Oculatellacean genera of these four strains, Gansulinema gen. nov. and Komarkovaeasiopsis gen. nov., are described based on a polyphasic approach. The ultrastructure of these strains showed a similar arrangement of peripheral thylakoids with three to four parallel layers, indicating that they belonged to the orders Nodosilineales, Oculatellales, or Leptolyngbyales. In the 16S rRNA gene phylogeny, two sequences of the Gansulinema strains and the two sequences of the Komarkovaeasiopsis strains formed two independent and robust clusters, within the order Oculatellales. The 16S rRNA gene sequences of strains of Komarkovaeasiopsis and Gansulinema showed low identity to each other (≤93.2%) and to other sequences of the Oculatellacean genera (≤94.5% and ≤93.3%, respectively). Furthermore, the 16S-23S internal transcribed spacer rRNA region secondary structures of strains of Komarkovaeasiopsis and Gansulinema were not consistent with all existing descriptions of Oculatellacean taxa. These data suggest that cyanobacterial communities are rich sources of new taxa in under-exploited areas, such as desert soils and hot spring in China.
为了增进对恶劣环境地区简单丝状蓝藻的了解，我们之前从中国沙漠土壤和温泉中分离和鉴定了四株（XN101、XN102、GS121、NX122）。结果，基于多相方法，描述了这四株中的两个新的 Oculatellacean 属，Gansulinema gen. nov. 和 Komarkovaeasiopsis gen. nov.。这些菌株的超微结构显示出外周类囊体的相似排列，有三到四层平行层，表明它们属于 Nodosilineales、Oculatellales 或 Leptolyngbyales 目。在 16S rRNA 基因系统发育中，两个 Gansulinema 菌株的序列和两个 Komarkovaeasiopsis 菌株的序列形成了两个独立且稳健的聚类，属于 Oculatellales 目。Komarkovaeasiopsis 和 Gansulinema 菌株的 16S rRNA 基因序列彼此之间的相似度较低（≤93.2%），与 Oculatellacean 属的其他序列的相似度也较低（≤94.5%和≤93.3%）。此外，Komarkovaeasiopsis 和 Gansulinema 菌株的 16S-23S 内部转录间隔区 rRNA 区域二级结构与所有现有的 Oculatellacean 分类群的描述不一致。这些数据表明，蓝藻群落是中国未充分开发的地区（如沙漠土壤和温泉）中新分类群的丰富来源。
