Faculdade de Ciências e Tecnologia da Universidade dos Açores, Ponta Delgada, Portugal.
CIBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, InBIO Laboratório Associado, BIOPOLIS Program in Genomics, Biodiversity and Land Planning; UNESCO Chair - Land Within Sea: Biodiversity & Sustainability in Atlantic Islands, Universidade dos Açores, Ponta Delgada, Portugal.
J Phycol. 2023 Dec;59(6):1323-1338. doi: 10.1111/jpy.13396. Epub 2023 Oct 16.
Simple filamentous cyanobacteria comprise a diverse and polyphyletic group of species, primarily in the orders Leptolyngbyales and Oscillatoriales, that need more sampling to improve their taxonomy. Oceanic islands, such as the Azores archipelago, present unique habitats and biogeographic conditions that harbor an unknown range of diversity of microorganisms. Filamentous cyanobacteria isolated from aquatic habitats in the Azores and maintained in the BACA culture collection were described using morphology, both light and transmission electron microscopy, ecology, and genetic data of the 16S rRNA gene sequences and 16S-23S Internal Transcribed Spacer (ITS) rRNA region secondary structure. Our analyses revealed two new monophyletic genera: Tumidithrix elongata gen. sp. nov. (Pseudanabaenaceae) and Radiculonema aquaticum gen. sp. nov. (Leptolyngbyaceae). In addition, two new species Leptodesmis lacustris sp. nov. (Leptolyngbyaceae) and Pycnacronema lacustrum sp. nov. (Wilmottiaceae) are reported as the first aquatic species for these genera. The description of these new taxa and the genetic study of an isolate of Leptodesmis alaskaensis from the Azores followed the polyphasic approach, identifying diacritical features. Our results reinforce the need for taxonomic studies on cyanobacteria from less-studied habits and geographic regions, which have a potential for new taxa description.
简单的丝状蓝藻包括一个多样化和多系的物种群体，主要在鞘丝藻目和颤藻目中，需要更多的采样来改善它们的分类。海洋岛屿，如亚速尔群岛，具有独特的生境和生物地理条件，蕴藏着未知范围的微生物多样性。从亚速尔群岛水生栖息地分离并保存在 BACA 培养物集中的丝状蓝藻，通过形态学、光学和透射电子显微镜、生态学以及 16S rRNA 基因序列和 16S-23S 内部转录间隔区（ITS）rRNA 区二级结构的遗传数据进行了描述。我们的分析揭示了两个新的单系属：Tumidithrix elongata gen. sp. nov.（拟鱼腥藻科）和 Radiculonema aquaticum gen. sp. nov.（鞘丝藻科）。此外，还报道了两个新种 Leptodesmis lacustris sp. nov.（鞘丝藻科）和 Pycnacronema lacustrum sp. nov.（Wilmottiaceae），它们是这些属的第一个水生种。这些新分类群的描述以及来自亚速尔群岛的 Leptodesmis alaskaensis 分离株的遗传研究遵循多相方法，确定了区别特征。我们的结果强调了对较少研究的生境和地理区域的蓝藻进行分类学研究的必要性，这些区域有可能描述新的分类群。
