Department of Fundamental and Applied Neurobiology, V. P. Serbsky National Medical Research Center of Psychiatry and Narcology, Ministry of Health of the Russian Federation, Moscow, Russia.
Bull Exp Biol Med. 2023 Dec;176(2):253-259. doi: 10.1007/s10517-024-06005-2. Epub 2024 Jan 10.
We studied the effect of exosomes derived from mesenchymal stem cells on the synthesis of collagen I and α-smooth muscle actin (α-SMA) by rat fibroblast culture. Exosomes were isolated from the verified culture of mesenchymal stem cells and also verified. Fibrosis was modeled using a fibroblast culture supplemented with recombinant TGF-β1 (5 ng/ml) and immunocytochemical analysis of the expression of collagen I and α-SMA markers was carried out. After 6-day incubation, the expression of the studied markers increased in comparison with the control. Addition of exosomes to the fibroblast culture reduced the production of collagen and SMA, which allows considering exosomes as a promising drug for the treatment of pathologies associated with fibrosis.
我们研究了间充质干细胞来源的外泌体对大鼠成纤维细胞培养中 I 型胶原和α-平滑肌肌动蛋白（α-SMA）合成的影响。外泌体是从已验证的间充质干细胞培养物中分离出来的，并进行了验证。使用补充有重组 TGF-β1（5ng/ml）的成纤维细胞培养物来建立纤维化模型，并进行 I 型胶原和α-SMA 标志物表达的免疫细胞化学分析。孵育 6 天后，与对照组相比，研究标记物的表达增加。将外泌体添加到成纤维细胞培养物中会减少胶原和 SMA 的产生，这使得外泌体有望成为治疗与纤维化相关的疾病的药物。
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