DNA G-四链体调节基因表型特异性分析指南。

Guide for phenotype-specific profiling of DNA G-quadruplex-regulated genes.

机构信息

Guangdong Provincial Key Laboratory of Colorectal and Pelvic Floor Diseases, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong 510655, China; Guangdong Institute of Gastroenterology, Guangzhou, Guangdong 510655, China; Department of Spine Surgery, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, China; Guangdong Province Key Laboratory of Orthopedics and Traumatology, Guangzhou 510080, China.

Department of Spine Surgery, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, China; Guangdong Province Key Laboratory of Orthopedics and Traumatology, Guangzhou 510080, China.

出版信息

STAR Protoc. 2024 Mar 15;5(1):102820. doi: 10.1016/j.xpro.2023.102820. Epub 2024 Jan 9.

DOI:10.1016/j.xpro.2023.102820

PMID:38198280

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10820308/

Abstract

DNA G-quadruplex (G4) is a non-canonical four-stranded secondary structure that has been shown to play a role in epigenetic modulation of gene expression. Here, we present a primer on phenotype-specific profiling of DNA G-quadruplex-regulated genes. We provide guidance on in silico exploration of G4-related genes and phenotypes, and in vitro and in vivo validation of the relationship between G4 and phenotype. We describe commonly utilized techniques and detail critical steps involved in determining the phenotype-specific G4-regulated genes for subsequent investigations.

摘要

DNA 四链体 (G4) 是一种非 canonical 的四级结构，已被证明在基因表达的表观遗传调控中发挥作用。在这里，我们提供了一个关于 DNA G4 调节基因表型特异性分析的入门指南。我们提供了关于 G4 相关基因和表型的计算机探索以及 G4 与表型之间关系的体内和体外验证的指导。我们描述了常用的技术，并详细说明了确定随后研究中表型特异性 G4 调节基因的关键步骤。