Alman B, Frasca P
J Orthop Res. 1987;5(1):139-43. doi: 10.1002/jor.1100050118.
This investigation examines the failure mechanism of bone from patients with osteogenesis imperfecta (OI). Mechanical testing and ultrastructural analysis of surgically obtained bone specimens, from patients with OI and from approximately age matched normal controls, were performed. Small rectangular bone samples (1.0 mm X 1.2 mm X 15 mm) with a prismatic cross section were put under three-point bending to determine the work to fracture. Furthermore, the fracture surface was observed with scanning electron microscopy (SEM). The SEM observation of partially dissected lamellae was also performed to determine the collagen organization of the bone. The OI bone has a consistently lower work to fracture and reduced collagen organization than the normal controls. The fracture surface of the OI bone is rough and exhibits a "pull-out" type of morphology, as opposed to the smoother fracture surface of the controls. This study shows that a relatively simple mechanical test utilizing a small amount of bone can differentiate between a diseased state (OI) and a normal state of bone.
本研究探讨了成骨不全症（OI）患者骨骼的失效机制。对手术获取的OI患者及年龄匹配的正常对照者的骨标本进行了力学测试和超微结构分析。将具有棱柱形横截面的小矩形骨样本（1.0毫米×1.2毫米×15毫米）进行三点弯曲以确定骨折功。此外，用扫描电子显微镜（SEM）观察骨折表面。还对部分解剖的薄片进行了SEM观察以确定骨的胶原组织。与正常对照相比，OI骨的骨折功始终较低，胶原组织减少。OI骨的骨折表面粗糙，呈现“拔出”型形态，而对照的骨折表面较光滑。本研究表明，利用少量骨进行的相对简单的力学测试可以区分患病状态（OI）和正常骨状态。
