Department of Microbiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.
Department of Microbiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.
Comp Immunol Microbiol Infect Dis. 2024 Feb;105:102125. doi: 10.1016/j.cimid.2024.102125. Epub 2024 Jan 6.
The aim of this study was to investigate the presence and genetic characteristics of Bartonella quintana in pet cats from Urmia City, located in the northwest of Iran. Blood samples were collected from 200 cats, and their age, gender, and breed were noted. Nested-PCR and sequencing were used to identify B. quintana in positive samples, and the ftsZ gene sequences were analyzed using BioEdit software. The gene sequence obtained in this study exhibited 100.00 % similarity to reference sequences in the GenBank® database, and a phylogenetic tree was constructed using MEGA11. The results revealed that 15 % of the cats (30 out of 200 blood samples) tested positive for the B. quintana gene, with a 95 % confidence interval of 10.71 % to 20.61 %.
本研究旨在调查来自伊朗西北部乌尔米耶市的宠物猫中是否存在巴尔通体五氏亚种（Bartonella quintana）及其遗传特征。采集了 200 只猫的血液样本，并记录了它们的年龄、性别和品种。采用巢式 PCR 和测序方法鉴定阳性样本中的 B. quintana，并使用 BioEdit 软件分析 ftsZ 基因序列。本研究获得的基因序列与 GenBank®数据库中的参考序列具有 100.00%的相似度，并使用 MEGA11 构建了系统发育树。结果表明，200 份血样中有 15%（30 份）检测到 B. quintana 基因阳性，置信区间为 10.71%至 20.61%。
