Guangxi Key Laboratory of Natural Polymer Chemistry and Physics, Nanning Normal University, Nanning, 530001, PR China.
Guangxi Key Laboratory of Natural Polymer Chemistry and Physics, Nanning Normal University, Nanning, 530001, PR China.
Talanta. 2024 Apr 1;270:125652. doi: 10.1016/j.talanta.2024.125652. Epub 2024 Jan 8.
Monitoring endogenous glutathione (GSH) levels in living cells is essential for cancer diagnose and treatment. In this work, GSH responsive fluorescent nanoprobe with turn-on property was constructed using Zn-modified porphyrinic metal-organic frameworks (PCN-224-Zn). The introduced Zn could quench the fluorescence of PCN-224 by the metallization of organic ligand (TCPP) and serves as sensing site for GSH. When exposed to GSH, the strong binding affinity of GSH generates the formation of Zn-GSH complex, eliminating the fluorescence quenching effect of Zn. Based on the constructed PCN-224-Zn nanoprobe, selective determination of GSH was achieved in the range of 0.01-6 μM with a detection limit of 1.5 nM. Furthermore, the constructed nanoprobe can realize the fluorescence imaging of endogenous GSH in MCF-7 and HeLa cells. Meanwhile, PCN-224-Zn could also monitor GSH in cell lysate with recovery rates from 93.8 % to 102.3 %. The performance of PCN-224-Zn demonstrates its capacities in the application of fluorescence sensing and bio-imaging fields.
监测活细胞内内源性谷胱甘肽 (GSH) 水平对于癌症的诊断和治疗至关重要。在这项工作中，使用 Zn 修饰的卟啉金属-有机骨架 (PCN-224-Zn) 构建了具有开启特性的 GSH 响应荧光纳米探针。引入的 Zn 可以通过有机配体 (TCPP) 的金属化来猝灭 PCN-224 的荧光，并作为 GSH 的传感位点。当暴露于 GSH 时，GSH 的强结合亲和力会生成 Zn-GSH 配合物，消除 Zn 的荧光猝灭效应。基于构建的 PCN-224-Zn 纳米探针，在 0.01-6 μM 的范围内实现了对 GSH 的选择性测定，检测限为 1.5 nM。此外，构建的纳米探针可以实现 MCF-7 和 HeLa 细胞内源性 GSH 的荧光成像。同时，PCN-224-Zn 还可以监测细胞裂解液中的 GSH，回收率从 93.8%到 102.3%不等。PCN-224-Zn 的性能证明了其在荧光传感和生物成像领域的应用潜力。
