Department of Biomedical Engineering, The Hong Kong Polytechnic University, Hung Hom, Kowloon 999077, Hong Kong SAR, PR China.
Institute of Nuclear Physics and Chemistry, China Academy of Engineering Physics (CAEP), Mianyang 612900, PR China.
ACS Appl Mater Interfaces. 2020 Jun 10;12(23):26391-26398. doi: 10.1021/acsami.0c06057. Epub 2020 May 26.
Herein, a dual-modal fluorescent/colorimetric "Signal-On" nanoprobe based on PCN-222 nanorods (NRs) toward phosphate was proposed for the first time. Due to the high affinity of the zirconium node in PCN-222 NRs for phosphate, the structure collapse of PCN-222 NRs was triggered by phosphate, resulting in the release of the tetrakis(4-carboxyphenyl)porphyrin (TCPP) ligand from PCN-222 NRs as well as the enhancement of fluorescence and absorbance signals. The PCN-222 NR-based nanoprobe could be employed for phosphate detection over a wide concentration range with a detection limit down to 23 nM. The practical application of the PCN-222 NR-based nanoprobe in real samples was evaluated. Moreover, benefitting from the good biocompatibility and water dispersibility of PCN-222 NRs, this nanoprobe was successfully employed in the intracellular imaging of phosphate, revealing its promising application in the biological science. The present work would greatly extend the potential of nanostructured MOFs in the sensing and biological fields.
本文首次提出了一种基于 PCN-222 纳米棒（NRs）的双模式荧光/比色“开启型”磷探针。由于 PCN-222 NRs 中锆节点对磷酸盐具有高亲和力，磷酸盐会触发 PCN-222 NRs 的结构崩溃，导致 PCN-222 NRs 中四（4-羧基苯基）卟啉（TCPP）配体的释放，同时增强荧光和吸收信号。基于 PCN-222 NR 的纳米探针可用于检测宽浓度范围内的磷酸盐，检测限低至 23 nM。评估了基于 PCN-222 NR 的纳米探针在实际样品中的实际应用。此外，由于 PCN-222 NRs 具有良好的生物相容性和水分散性，该纳米探针成功地用于磷酸盐的细胞内成像，显示出其在生物科学中的应用前景。本工作将极大地扩展纳米结构 MOFs 在传感和生物领域的潜力。
