Cunningham I, Taylor A M
J Protozool. 1979 Aug;26(3):428-32. doi: 10.1111/j.1550-7408.1979.tb04649.x.
When transformed procyclic noninfective trypanosomes of several unrelated stocks of Trypanosoma brucei were cultivated in T-30 Falcon flasks at 28 C in a liquid medium containing head-salivary gland explants of Glossina morsitans morsitans some of the organisms developed into forms infective for mice. Infective trypanosomes were detected 7 to 14 days after the cultures were prepared and they persisted for varying periods of up to 88 days when the cultures were terminated. A few of the salivary glands became invaded with parasites about the time infective organisms appeared in the cultures. Using T. brucei TREU 929, it was shown that trypanosomes grown with between 2m and 50 explants were capable of producing infections consistently for prolonged periods. On the other hand, trypanosomes cultivated with 25 or fewer explants rarely infected mice. Infectivity titrations on trypanosome suspensions from cultures of stocks TREU 1275 and TREU 929 revealed that the maximum number of infective organisms was present 26 to 50 days after initiation of the cultures. Control cultures of trypanosomes grown in medium alone were generally not infective but 2 of the 6 stocks gave rise to a few sporadic infections. A few epimastigote-like and metacyclic-like trypanosomes were seen in stained preparations of infective inocula.
当几种无关的布氏锥虫原循环非感染性锥虫株在28℃下于含有 morsitans morsitans 采采蝇头部唾液腺外植体的液体培养基中在T-30 Falcon培养瓶中培养时，一些生物体发育成可感染小鼠的形式。在制备培养物7至14天后检测到感染性锥虫，当培养物终止时，它们持续不同时间长达88天。在培养物中出现感染性生物体时，一些唾液腺大约在此时被寄生虫侵入。使用布氏锥虫TREU 929，结果表明，与2个至50个外植体一起培养的锥虫能够长时间持续产生感染。另一方面，用25个或更少外植体培养的锥虫很少感染小鼠。对来自TREU 1275和TREU 929株培养物的锥虫悬液进行感染性滴定，结果显示，在培养开始后26至50天存在的感染性生物体数量最多。仅在培养基中生长的锥虫对照培养物通常没有感染性，但6个株系中的2个导致了一些零星感染。在感染性接种物的染色制剂中可见一些类上鞭毛体和类循环后期锥虫。
