Department of Biomedical Science and Engineering, Gwangju Institute of Science and Technology, Gwangju, South Korea.
J Sleep Res. 2024 Oct;33(5):e14137. doi: 10.1111/jsr.14137. Epub 2024 Jan 10.
The association between sleep and pain has been investigated widely. However, inconsistent results from animal studies compared with human data show the need for a validated animal model in the sleep-pain association field. Our study aims to validate common neuropathic pain models as a tool for evaluating the sleep-pain association. Electrodes electroencephalogram (EEG) and electromyogram (EMG) were surgically implanted to measure sleep. The von Frey test was used to measure pain sensitivity. Following the baseline data acquisition, two pain-modelling procedures were performed: sciatic nerve crush injury (SCI) and common peroneal nerve ligation (CPL). Post-injury measurements were performed on days 1, 5, 10, and 15 post-surgery. The results presented decreased paw withdrawal thresholds and reduced NREM sleep duration in both models on the first post-surgery day. In the SCI model, NREM sleep duration was negatively correlated with paw withdrawal thresholds (p = 0.0466), but not in the CPL model. Wake alpha and theta EEG powers were also correlated with the pain threshold. The results confirm that the SCI model shows disturbed sleep patterns associated with increased pain sensitivity, suggesting it is a reliable tool for investigating sleep disturbances associated with neuropathic pain.
睡眠与疼痛之间的关系已经得到了广泛的研究。然而，动物研究与人类数据的结果不一致，这表明需要在睡眠-疼痛关联领域中验证一种经过验证的动物模型。我们的研究旨在验证常见的神经病理性疼痛模型，作为评估睡眠-疼痛关联的工具。通过手术植入电极脑电图（EEG）和肌电图（EMG）来测量睡眠。使用 von Frey 测试来测量疼痛敏感性。在获得基线数据后，进行了两种疼痛建模程序：坐骨神经挤压伤（SCI）和腓总神经结扎（CPL）。手术后第 1、5、10 和 15 天进行损伤后测量。结果表明，两种模型在手术后的第一天，即出现了爪回缩阈值降低和非快速眼动睡眠（NREM）持续时间减少。在 SCI 模型中，NREM 睡眠持续时间与爪回缩阈值呈负相关（p=0.0466），但在 CPL 模型中则没有。清醒时α和θ脑电功率也与疼痛阈值相关。研究结果证实，SCI 模型显示出与疼痛敏感性增加相关的睡眠模式紊乱，表明它是研究与神经病理性疼痛相关的睡眠障碍的可靠工具。
