Flavor profiling and gene expression studies of indigenous aromatic rice variety (Mushk Budiji) grown at different altitudes of Highland Himalayan regions.

Mushk Budiji-an indigenous aromatic rice variety is usually grown at an altitude ranging from 5000 to 7000 ft above mean sea level in Highland Himalayas. This study was conducted to investigate the effects of altitude, soil nitrogen content and climatic conditions (temperature) of the selected locations on the flavor profile of Mushk Budiji using gas chromatography-mass spectroscopy (GC-MS) and electronic nose (E-nose). E-nose being rapid and non-destructive method was used to validate the results of volatile aromatic compounds obtained using GC-MS in Mushk Budiji. Around 35 aromatic compounds were identified in Mushk Budiji rice samples. Highest volatile peak area percentage (105.41%) was recorded for Mushk Budji grown at an altitude of 5216.53 ft. Highest E-nose score (2.52) was obtained at an altitude of 6299.21 ft. Over-expression of fatty acid degradation and linoleic acid metabolism genes was observed at higher altitudes, whereas lipid biosynthesis was negatively influenced by higher altitude. Fatty acid degradation and linoleic acid metabolism is responsible for the synthesis of volatile aromatic compounds in Mushk Budiji. This study will therefore be the path finder for investigating the intricate mechanism behind the role of altitude on aroma development in Mushk Budiji rice for future studies.