The Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China.
State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China.
FEMS Microbiol Lett. 2024 Jan 9;371. doi: 10.1093/femsle/fnae002.
CrgA has been shown to be a negative regulator of carotenogenesis in some filamentous fungi, while light irradiation is an inducible environmental factor for carotenoid biosynthesis. To clarify the relationship between CrgA and light-inducible carotenogenesis in Blakeslea trispora, the cis-acting elements of the btcrgA promoter region were investigated, followed by the analyses of correlation between the expression of btcrgA and carotenoid structural genes under different irradiation conditions. A variety of cis-acting elements associated with light response was observed in the promoter region of btcrgA, and transcription of btcrgA and carotenoid structural genes under different irradiation conditions was induced by white light with a clear correlation. Then, RNA interference and overexpression of btcrgA were performed to investigate their effects on carotenogenesis at different levels under irradiation and darkness. The analyses of transcription and enzyme activities of carotenoid structural gene, and accumulation of carotenoids among btcrgA-interfered, btcrgA-overexpressed, and wild-type strains under irradiation and darkness indicate that btcrgA negatively regulates the synthesis of carotenoid in darkness, while promotes the carotenogenesis under irradiation regardless of reduced or overexpression of btcrgA .
CrgA 已被证明是一些丝状真菌类胡萝卜素生物合成的负调控因子，而光照是类胡萝卜素生物合成的诱导环境因素。为了阐明布雷德福假丝酵母（Blakeslea trispora）中 CrgA 与光诱导类胡萝卜素生物合成之间的关系，研究了 btcrgA 启动子区域的顺式作用元件，并分析了在不同光照条件下 btcrgA 和类胡萝卜素结构基因表达之间的相关性。在 btcrgA 的启动子区域观察到与光反应相关的多种顺式作用元件，btcrgA 和类胡萝卜素结构基因在不同光照条件下的转录均被白光诱导，相关性明显。然后，通过 RNA 干扰和 btcrgA 的过表达，在光照和黑暗条件下从不同水平研究了它们对类胡萝卜素生物合成的影响。在光照和黑暗条件下，对 btcrgA 干扰、btcrgA 过表达和野生型菌株的类胡萝卜素结构基因转录和酶活性以及类胡萝卜素积累的分析表明，btcrgA 在黑暗中负调控类胡萝卜素的合成，而在光照下促进类胡萝卜素的生物合成，无论 btcrgA 的表达减少还是过表达。
