Zheng Qiang, Zhu Kaili, Wang Ke, Wang Yi, Yu Xiaobin, Luo Wei
Modern Industrial College of Traditional Chinese Medicine and Health, Lishui University, Lishui, China.
The Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, China.
Front Microbiol. 2025 Mar 25;16:1554367. doi: 10.3389/fmicb.2025.1554367. eCollection 2025.
β-Carotene, a high value-added natural pigment, is currently produced industrially in . Although photoinduced carotenoid synthesis has been identified in some filamentous fungi, there are still relatively few studies focusing on and its potential mechanisms. In this study, an integrated strategy-including correlation analysis of gene expression, bioinformatics analysis, protein interaction, and RNA interference-was adopted to elucidate photoinduced β-carotene synthesis in . Light wavelength, intensity, and irradiation duration stimulated the transcription of photoreceptors [ () and ()] and carotenoid structural genes ( and ). The transcription of photoreceptor genes showed significant or high correlation with carotenoid structural genes under continuous or short-term, high-intensity blue light irradiation. To elucidate the role of photoreceptors in carotenoid synthesis, the interaction between BTWC-1 and BTWC-2 was predicted. Furthermore, Glutathione S-Transferase (GST) pull-down assays showed that only BTWC-1C and BTWC-2A could interact to form complexes. Inhibition of expression under dark conditions did not affect β-carotene accumulation or the transcription of and , but did reduce these parameters under blue light irradiation, indicating that mediates photoinduced β-carotene synthesis in .
β-胡萝卜素是一种高附加值的天然色素，目前在工业上进行生产。尽管在一些丝状真菌中已发现光诱导类胡萝卜素合成，但针对其及潜在机制的研究仍相对较少。在本研究中，采用了一种综合策略，包括基因表达的相关性分析、生物信息学分析、蛋白质相互作用和RNA干扰，以阐明[具体对象未提及]中的光诱导β-胡萝卜素合成。光波长、强度和照射持续时间刺激了光感受器[（具体名称未提及）和（具体名称未提及）]以及类胡萝卜素结构基因（具体基因名称未提及）和（具体基因名称未提及）的转录。在连续或短期、高强度蓝光照射下，光感受器基因的转录与类胡萝卜素结构基因显示出显著或高度相关性。为了阐明光感受器在类胡萝卜素合成中的作用，预测了BTWC-1和BTWC-2之间的相互作用。此外，谷胱甘肽S-转移酶（GST）下拉试验表明，只有BTWC-1C和BTWC-2A能够相互作用形成复合物。在黑暗条件下抑制[具体基因名称未提及]的表达不影响β-胡萝卜素积累或[具体基因名称未提及]和[具体基因名称未提及]的转录，但在蓝光照射下会降低这些参数，表明[具体基因名称未提及]介导了[具体对象未提及]中的光诱导β-胡萝卜素合成。
