Wang Xinrong, Yu Haidong, Zhang Wenli, Fu Lizhi, Wang Yue
College of Veterinary Medicine, Southwest University, Chongqing 400715, China.
Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150068, China.
Animals (Basel). 2023 Dec 19;14(1):6. doi: 10.3390/ani14010006.
To investigate the distribution and genetic variation in four vertically transmitted duck pathogens, including duck hepatitis B virus (DHBV), duck circovirus (DuCV), duck hepatitis A virus 3 (DHAV-3), and avian reoviruses (ARV), we conducted an epidemiology study using PCR and RT-PCR assays on a duck population. We found that DHBV was the most prevalent virus (69.74%), followed by DuCV (39.48%), and then ARV (19.92%) and DHAV-3 (8.49%). Among the 271 duck samples, two, three or four viruses were detected in the same samples, indicating that the coinfection of vertical transmission agents is common in ducks. The genetic analysis results showed that all four identified DuCV strains belonged to genotype 1, the DHAV-3 strain was closely clustered with previously identified strains from China, and the ARV stain was clustered under genotype 1. These indicate that different viral strains are circulating among the ducks. Our findings will improve the knowledge of the evolution of DuCV, DHAV-3, and ARV, and help choose suitable strains for vaccination.
为了研究四种垂直传播的鸭病原体，包括鸭乙型肝炎病毒（DHBV）、鸭圆环病毒（DuCV）、鸭甲型肝炎病毒3型（DHAV-3）和禽呼肠孤病毒（ARV）的分布及基因变异情况，我们对一群鸭子进行了一项流行病学研究，采用聚合酶链反应（PCR）和逆转录聚合酶链反应（RT-PCR）检测方法。我们发现DHBV是最普遍的病毒（69.74%），其次是DuCV（39.48%），然后是ARV（19.92%）和DHAV-3（8.49%）。在271份鸭样本中，同一样本检测到两种、三种或四种病毒，这表明垂直传播病原体的共感染在鸭子中很常见。基因分析结果显示，所有鉴定出的DuCV毒株均属于1型基因型，DHAV-3毒株与之前在中国鉴定出的毒株紧密聚类，ARV毒株聚类在1型基因型下。这些表明不同的病毒株在鸭子中传播。我们的研究结果将增进对DuCV、DHAV-3和ARV进化的了解，并有助于选择合适的毒株用于疫苗接种。
