昆虫单细胞水平的研究进展：技术与应用

Insect Insights at the Single-Cell Level: Technologies and Applications.

机构信息

Analysis Center of Agrobiology and Environmental Sciences, Zhejiang University, Hangzhou 310058, China.

Institute of Sericulture and Apiculture, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China.

出版信息

Cells. 2023 Dec 31;13(1):91. doi: 10.3390/cells13010091.

DOI:10.3390/cells13010091

PMID:38201295

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10777908/

Abstract

Single-cell techniques are a promising way to unravel the complexity and heterogeneity of transcripts at the cellular level and to reveal the composition of different cell types and functions in a tissue or organ. In recent years, advances in single-cell RNA sequencing (scRNA-seq) have further changed our view of biological systems. The application of scRNA-seq in insects enables the comprehensive characterization of both common and rare cell types and cell states, the discovery of new cell types, and revealing how cell types relate to each other. The recent application of scRNA-seq techniques to insect tissues has led to a number of exciting discoveries. Here we provide an overview of scRNA-seq and its application in insect research, focusing on biological applications, current challenges, and future opportunities to make new discoveries with scRNA-seq in insects.

摘要

单细胞技术是一种有前途的方法，可以揭示细胞水平转录本的复杂性和异质性，并揭示组织或器官中不同细胞类型和功能的组成。近年来，单细胞 RNA 测序 (scRNA-seq) 的进展进一步改变了我们对生物系统的看法。scRNA-seq 在昆虫中的应用使我们能够全面描述常见和罕见的细胞类型和细胞状态，发现新的细胞类型，并揭示细胞类型之间的关系。最近将 scRNA-seq 技术应用于昆虫组织已经带来了一些令人兴奋的发现。在这里，我们提供了 scRNA-seq 的概述及其在昆虫研究中的应用，重点介绍了生物学应用、当前的挑战以及未来利用 scRNA-seq 在昆虫中进行新发现的机会。