Loyo Carlos, Cordoba Alexander, Palza Humberto, Canales Daniel, Melo Francisco, Vivanco Juan F, Baier Raúl Vallejos, Millán Carola, Corrales Teresa, Zapata Paula A
Departamento de Ciencias del Ambiente, Facultad de Química y Biología, Universidad de Santiago de Chile (USACH), Grupo Polímeros, Santiago 9160000, Chile.
School of Chemical Sciences and Engineering, Yachay Tech University, Hda. San José s/n y Proyecto Yachay, Urcuquí 100119, Ecuador.
Polymers (Basel). 2023 Dec 30;16(1):129. doi: 10.3390/polym16010129.
Polymer-based nanocomposites such as polycaprolactone/graphene oxide (PCL/GO) have emerged as alternatives for bone tissue engineering (BTE) applications. The objective of this research was to investigate the impact of a gelatin (Gt) coating on the degradability and different properties of PCL nanofibrous scaffolds fabricated by an electrospinning technique with 1 and 2 wt% GO. Uniform PCL/GO fibers were obtained with a beadless structure and rough surface. PCL/GO scaffolds exhibited an increase in their crystallization temperature (Tc), attributed to GO, which acted as a nucleation agent. Young's modulus increased by 32 and 63% for the incorporation of 1 and 2 wt% GO, respectively, in comparison with neat PCL. A homogeneous Gt coating was further applied to these fibers, with incorporations as high as 24.7 wt%. The introduction of the Gt coating improved the hydrophilicity and degradability of the scaffolds. Bioactivity analysis revealed that the hydroxyapatite crystals were deposited on the Gt-coated scaffolds, which made them different from their uncoated counterparts. Our results showed the synergic effect of Gt and GO in enhancing the multifunctionality of the PCL, in particular the degradability rate, bioactivity, and cell adhesion and proliferation of hGMSC cells, making it an interesting biomaterial for BTE.
基于聚合物的纳米复合材料，如聚己内酯/氧化石墨烯（PCL/GO），已成为骨组织工程（BTE）应用的替代材料。本研究的目的是研究明胶（Gt）涂层对通过静电纺丝技术制备的含1 wt%和2 wt% GO的PCL纳米纤维支架的降解性和不同性能的影响。获得了具有无珠结构和粗糙表面的均匀PCL/GO纤维。PCL/GO支架的结晶温度（Tc）升高，这归因于起成核剂作用的GO。与纯PCL相比，加入1 wt%和2 wt% GO后，杨氏模量分别提高了32%和63%。进一步对这些纤维施加均匀的Gt涂层，其掺入量高达24.7 wt%。Gt涂层的引入改善了支架的亲水性和降解性。生物活性分析表明，羟基磷灰石晶体沉积在Gt涂层支架上，这使其与未涂层的支架有所不同。我们的结果显示了Gt和GO在增强PCL多功能性方面的协同作用，特别是在降解速率、生物活性以及人牙龈间充质干细胞（hGMSC）的细胞粘附和增殖方面，使其成为一种用于BTE的有趣生物材料。
