School of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, China.
Division of Natural and Applied Sciences, Duke Kunshan University, Kunshan 215316, China.
Nutrients. 2023 Dec 19;16(1):8. doi: 10.3390/nu16010008.
In an era of increasing interest in the potential health benefits of medicinal foods, the need to assess their safety and potential toxicity remains a critical concern. While these natural remedies have garnered substantial attention for their therapeutic potential, a comprehensive understanding of their effects on living organisms is essential. We examined 316 herbal extracts to determine their potential nematocidal attributes in . Approximately 16% of these extracts exhibited the capacity to induce diminished survival rates and larval arrest, establishing a correlation between larval arrest and overall worm viability. Certain extracts led to an unexpected increase in male nematodes, accompanied by a discernible reduction in DAPI-stained bivalent structures and perturbed meiotic advancement, thereby disrupting the conventional developmental processes. Notably, and extracts activated a DNA damage checkpoint response via the ATM/ATR and CHK-1 pathways, thus hindering germline development. Our LC-MS analysis revealed jervine in and nine antitumor compounds in . Interestingly, linoleic acid replicated phenotypes induced by exposure, including an increased level of pCHK-1 foci, apoptosis, and the MAPK pathway. Mutants in the MAPK pathway mitigated the decline in worm survival, underscoring its importance in promoting worm viability. This study reveals complex interactions between herbal extracts and processes, shedding light on potential antitumor effects and mechanisms. The findings provide insights into the complex landscape of herbal medicine's impact on a model organism, offering implications for broader applications.
在人们越来越关注药用食品潜在健康益处的时代，评估其安全性和潜在毒性仍然是一个关键问题。虽然这些天然药物因其治疗潜力而引起了广泛关注，但全面了解它们对生物体的影响至关重要。我们研究了 316 种草药提取物，以确定它们在 中的潜在杀线虫特性。这些提取物中约有 16%具有诱导存活率降低和幼虫停滞的能力，这表明幼虫停滞与整体蠕虫活力之间存在相关性。某些提取物导致雄性线虫数量意外增加，同时双价体结构的 DAPI 染色明显减少，减数分裂过程受到干扰，从而破坏了常规的发育过程。值得注意的是，和 提取物通过 ATM/ATR 和 CHK-1 途径激活了 DNA 损伤检查点反应，从而阻碍了生殖细胞的发育。我们的 LC-MS 分析在 和 9 种抗肿瘤化合物中发现了 jervine。有趣的是，亚油酸复制了 暴露引起的表型，包括增加 pCHK-1 焦点、细胞凋亡和 MAPK 途径。MAPK 途径中的突变体减轻了线虫存活率的下降，突出了它在促进线虫活力方面的重要性。这项研究揭示了草药提取物与 过程之间的复杂相互作用，揭示了潜在的抗肿瘤作用和机制。这些发现为草药对模式生物的影响提供了更深入的了解，为更广泛的应用提供了启示。
