Department of Experimental Oncology, Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, 53-114 Wroclaw, Poland.
Nutrients. 2023 Dec 22;16(1):49. doi: 10.3390/nu16010049.
The hormonally active vitamin D metabolite, calcitriol, functions as an important modulator of the immune system. We assumed that calcitriol exerts different effects on immune cells and cytokine production, depending on the age of the animal; therefore, we analyzed its effects on regulatory T lymphocytes and regulatory B lymphocytes in healthy young and old female C57Bl/6/Foxp3GFP mice. In the lymph nodes of young mice, calcitriol decreased the percentage of Tregs, including tTregs and pTregs, and the expression of GITR, CD103, and CD101; however, calcitriol increased the level of IL-35 in adipose tissue. In the case of aged mice, calcitriol decreased the percentages of tTregs and CD19+ cells in lymph nodes and the level of osteopontin in the plasma. Additionally, increases in the levels of IgG and the lowest levels of IFN-γ, IL-10, and IL-35 were observed in the adipose tissue of aged mice. This study showed that calcitriol treatment had different effects, mainly on Treg phenotypes and cytokine secretion, in young and old female mice; it seemed that calcitriol enhanced the immunosuppressive properties of the lymphatic organs and adipose tissue of healthy young mice but not of healthy aged mice, where the opposite effects were observed.
具有生物活性的维生素 D 代谢产物 1,25-二羟维生素 D3（calcitriol）作为免疫系统的重要调节剂。我们假设 1,25-二羟维生素 D3 会根据动物年龄的不同对免疫细胞和细胞因子的产生产生不同的影响；因此，我们分析了其对健康年轻和老年雌性 C57Bl/6/Foxp3GFP 小鼠调节性 T 淋巴细胞和调节性 B 淋巴细胞的影响。在年轻小鼠的淋巴结中，1,25-二羟维生素 D3 降低了 Tregs（包括 tTregs 和 pTregs）和 GITR、CD103 和 CD101 的表达的百分比；然而，1,25-二羟维生素 D3 增加了脂肪组织中 IL-35 的水平。在老年小鼠的情况下，1,25-二羟维生素 D3 降低了淋巴结中 tTregs 和 CD19+细胞的百分比以及血浆中骨桥蛋白的水平。此外，在老年小鼠的脂肪组织中观察到 IgG 水平增加和 IFN-γ、IL-10 和 IL-35 水平最低。本研究表明，1,25-二羟维生素 D3 处理对年轻和老年雌性小鼠的 Treg 表型和细胞因子分泌有不同的影响；似乎 1,25-二羟维生素 D3 增强了健康年轻小鼠的淋巴器官和脂肪组织的免疫抑制特性，但对健康老年小鼠则没有，反而观察到相反的效果。
