Unidad de Investigación Biomédica, Delegación Zacatecas, México, Instituto Mexicano del Seguro Social, IMSS.
Unidad de Medicina Familiar Numero 4, Delegación Zacatecas, México, Instituto Mexicano del Seguro Social, IMSS.
Obes Res Clin Pract. 2018 Nov-Dec;12(6):513-519. doi: 10.1016/j.orcp.2018.07.001. Epub 2018 Aug 13.
Regulatory B (Breg) cells have a critical role in adipose tissue homeostasis, and although subtypes of Breg cells have been described, their contribution during obesity is unclear. Therefore, the levels of regulatory B cells in adipose tissue and peripheral blood samples drawn from individuals with overweight, obesity, and normal-weight were evaluated.
The percentages of Breg cells were analyzed by flow cytometry. The activity of Breg cells was assessed by measuring the release of IFN-γ in the supernatants of co-cultures of CD4+ T and regulatory B cells with an ELISA assay. The levels of IL-17 and IFN-γ produced by the CD4+ T cells were assessed using an ELISA assay.
Diminished frequencies of Breg cells with phenotypes CD19+CD27+CD38High, CD19+CD24HighCD38High, and CD19+CD24HighCD38HighIL-10+ cells were observed in the blood samples from the individuals with overweight and obesity but not in the individuals with normal-weight. The production of IFN-γ in CD4+ T-cell cultures showed a decrease in the presence of Breg cells in individuals with obesity and normal-weight. We found fewer percentages of CD19+CD27+CD38High cells in the adipose tissue samples from individuals with overweight and obesity compared to individuals with normal-weight. In addition, elevated levels of IL-17 and IFN-γ in the supernatants of the cultures of CD4+ T cells from the individuals with obesity compared to the individuals with normal-weight were observed.
The results suggest that individuals with obesity show increased levels of Th1/Th17 cytokines, which might be caused by the decreased frequency of regulatory B cells.
调节性 B (Breg) 细胞在脂肪组织稳态中具有关键作用，尽管已经描述了 Breg 细胞的亚型，但它们在肥胖中的作用尚不清楚。因此，评估了超重、肥胖和正常体重个体的脂肪组织和外周血样本中的调节性 B 细胞水平。
通过流式细胞术分析 Breg 细胞的百分比。通过 ELISA 测定共培养 CD4+T 和调节性 B 细胞释放 IFN-γ来评估 Breg 细胞的活性。通过 ELISA 测定 CD4+T 细胞产生的 IL-17 和 IFN-γ的水平。
与正常体重个体相比，超重和肥胖个体的血液样本中具有表型 CD19+CD27+CD38High、CD19+CD24HighCD38High 和 CD19+CD24HighCD38HighIL-10+的 Breg 细胞频率降低，但在正常体重个体中没有观察到这种情况。在肥胖和正常体重个体中，存在 Breg 细胞时，CD4+T 细胞培养物中 IFN-γ的产生减少。与正常体重个体相比，超重和肥胖个体的脂肪组织样本中 CD19+CD27+CD38High 细胞的百分比较少。此外，与正常体重个体相比，肥胖个体的 CD4+T 细胞培养物上清液中 IL-17 和 IFN-γ的水平升高。
这些结果表明，肥胖个体表现出 Th1/Th17 细胞因子水平升高，这可能是由于调节性 B 细胞频率降低所致。
