Lee C Y, Wong E, Zhang J H
J Reprod Immunol. 1986 Dec;9(4):261-74. doi: 10.1016/0165-0378(86)90028-8.
The inhibitory effects of monoclonal antibodies on the fertilization of mouse oocytes were evaluated in vitro and in vivo. Among the 40 sperm-specific monoclonal antibodies that had been examined, nine showed significant inhibition of the fertilization of mouse oocytes in vitro. MS 204 was shown to cause a high incidence of penetration of the zona pellucida of mouse eggs by multiple sperm, when the sperm concentration for insemination exceeds 1 X 10(5)/ml. This antibody prevented further penetration of the vitelline membrane by sperm. On the other hand, sperm penetration to zona was inhibited in the presence of MS 207. However, neither MS 204 nor MS 207 caused significant inhibition of penetration of zona-free mouse or hamster eggs by sperm. MS 204 and MS 207 were also found to inhibit the fertilization of mouse oocytes in vivo and embryo development in vitro, when superovulated mice were injected intraperitoneally with given doses of antibodies prior to the mating. Further in vitro culture of the recovered 2-cell oocytes revealed little or no further embryo development beyond two- to four-cell stages. In the controls, greater than 80% of the retrieved oocytes were fertilized and successively developed to the blastocyst stages in vitro when the ascites fluid from NS-1 cells was administered. Two of the monoclonal antibodies generated against human sperm antigens, HS 11 and HS 63, were shown to cross-react specifically with mouse sperm acrosomal antigens and also inhibited the fertilization of mouse oocytes in vitro and in vivo. The results of this study suggest that some monoclonal antibodies to sperm acrosomal antigens exhibit strongly inhibitory effects on the in vitro and in vivo fertilization of mouse oocytes as well as subsequent development of early embryos. As a comparative control, rabbit antisera against sperm-specific enzymes, lactate dehydrogenase-X and 3-phosphoglycerate kinase-2, showed little or no inhibition on the fertilization of mouse oocytes in vitro or in vivo or the subsequent embryo development.
在体外和体内评估了单克隆抗体对小鼠卵母细胞受精的抑制作用。在所检测的40种精子特异性单克隆抗体中，有9种在体外对小鼠卵母细胞受精显示出显著抑制作用。当授精的精子浓度超过1×10⁵/ml时，MS 204可导致多个精子穿透小鼠卵子透明带的发生率很高。该抗体可阻止精子进一步穿透卵黄膜。另一方面，在MS 207存在时精子对透明带的穿透受到抑制。然而，MS 204和MS 207均未对无透明带小鼠或仓鼠卵子的精子穿透产生显著抑制作用。当超排卵小鼠在交配前腹腔注射一定剂量的抗体时，还发现MS 204和MS 207可抑制小鼠卵母细胞的体内受精和体外胚胎发育。对回收的2细胞卵母细胞进行进一步体外培养发现，在二细胞至四细胞阶段之后几乎没有或没有进一步的胚胎发育。在对照组中，当给予NS-1细胞的腹水时，超过80%的回收卵母细胞受精并在体外相继发育至囊胚阶段。两种针对人精子抗原产生的单克隆抗体HS 11和HS 63被证明与小鼠精子顶体抗原发生特异性交叉反应，并且在体外和体内也抑制小鼠卵母细胞受精。本研究结果表明，一些针对精子顶体抗原的单克隆抗体对小鼠卵母细胞的体外和体内受精以及早期胚胎的后续发育具有强烈的抑制作用。作为比较对照，针对精子特异性酶乳酸脱氢酶-X和3-磷酸甘油酸激酶-2的兔抗血清在体外或体内对小鼠卵母细胞受精或随后的胚胎发育几乎没有抑制作用。
