National Soybean Processing Industry Technology Innovation Center, Beijing Technology and Business University, Beijing 100048, China.
Department of Nutrition and Health, China Agricultural University, Beijing 100193, China.
Nutrients. 2023 Dec 25;16(1):71. doi: 10.3390/nu16010071.
The occupational groups exposed to air pollutants, particularly PM2.5, are closely linked to the initiation and advancement of respiratory disorders. The aim of this study is to investigate the potential protective properties of selenium-enriched soybean peptides (Se-SPeps), a novel Se supplement, in mitigating apoptosis triggered by PM2.5 in A549 lung epithelial cells. The results indicate a concentration-dependent reduction in the viability of A549 cells caused by PM2.5, while Se-SPeps at concentrations of 62.5-500 µg/mL showed no significant effect. Additionally, the Se-SPeps reduced the production of ROS, proinflammatory cytokines, and apoptosis in response to PM2.5 exposure. The Se-SPeps suppressed the PM2.5-induced upregulation of Bax/Bcl-2 and caspase-3, while also restoring reductions in p-Akt in A549 cells. The antiapoptotic effects of Se-SPeps have been found to be more effective compared to SPeps, SeMet, and NaSeO when evaluated at an equivalent protein or Se concentration. Our study results furnish evidence that supports the role of Se-SPeps in reducing the harmful effects of PM2.5, particularly in relation to its effect on apoptosis, oxidative stress, and inflammation.
职业群体接触空气污染物，特别是 PM2.5，与呼吸疾病的发生和发展密切相关。本研究旨在探究富硒大豆肽（Se-SPeps）作为一种新型硒补充剂，对 PM2.5 诱导的 A549 肺上皮细胞凋亡的潜在保护作用。结果表明，PM2.5 浓度依赖性地降低了 A549 细胞的活力，而浓度为 62.5-500μg/ml 的 Se-SPeps 则没有显著影响。此外，Se-SPeps 减少了 ROS、促炎细胞因子和细胞凋亡的产生，以应对 PM2.5 的暴露。Se-SPeps 抑制了 PM2.5 诱导的 Bax/Bcl-2 和 caspase-3 的上调，同时恢复了 A549 细胞中 p-Akt 的减少。与 SPeps、SeMet 和 NaSeO 相比，在评估等效蛋白或 Se 浓度时，Se-SPeps 的抗凋亡作用更为有效。我们的研究结果为富硒大豆肽在减轻 PM2.5 有害影响方面的作用提供了证据，特别是在其对细胞凋亡、氧化应激和炎症的影响方面。
