Division of Biological Science, Graduate School of Science and Technology, Nara Institute of Science and Technology, 8916-5 Takayamacho, Ikoma 630-0192, Nara, Japan.
Institute for Research Initiatives, Nara Institute of Science and Technology, 8916-5 Takayamacho, Ikoma 630-0192, Nara, Japan.
Int J Mol Sci. 2023 Dec 25;25(1):304. doi: 10.3390/ijms25010304.
A cell population characterized by the release of glucose repression and known as [] emerges spontaneously in the yeast . This study revealed that the [] variants exhibit retarded alcoholic fermentation when glucose is the sole carbon source. To identify the key to the altered glucose response, the gene expression profile of [] cells was examined. Based on RNA-seq data, the [] status was linked to impaired function of the Cyc8p-Tup1p complex. Loss of Cyc8p led to a decrease in the initial rate of alcoholic fermentation under glucose-rich conditions via the inactivation of pyruvate decarboxylase, an enzyme unique to alcoholic fermentation. These results suggest that Cyc8p can become inactive to attenuate alcoholic fermentation. These findings may contribute to the elucidation of the mechanism of non-genetic heterogeneity in yeast alcoholic fermentation.
酵母中自发出现一种细胞群体，其特征是释放葡萄糖抑制作用，被称为 []。本研究表明，当葡萄糖是唯一碳源时，[] 变体表现出酒精发酵迟缓。为了确定改变葡萄糖反应的关键，研究人员检查了 [] 细胞的基因表达谱。基于 RNA-seq 数据，[] 状态与 Cyc8p-Tup1p 复合物功能受损有关。Cyc8p 的缺失导致在富葡萄糖条件下通过抑制丙酮酸脱羧酶（一种独特的酒精发酵酶）的活性，使酒精发酵的初始速率降低。这些结果表明，Cyc8p 可以失活以减弱酒精发酵。这些发现可能有助于阐明酵母酒精发酵中非遗传异质性的机制。
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