Rheumatology Unit, Department of Medicine-DIMED, University of Padova, 35128 Padova, Italy.
Medicine Unit 1, Ca' Foncello Hospital, 31100 Treviso, Italy.
Int J Mol Sci. 2023 Dec 27;25(1):393. doi: 10.3390/ijms25010393.
The role of calcium pyrophosphate (CPP) crystals in osteoarthritis (OA) is still a matter of debate. With this study we aimed to investigate the inflammatory features of synovial fluid (SF) collected from patients with OA with CPP crystals compared with those without crystals. We also explored the effect of OA SF on monocytes response. SFs were collected from adult patients with OA and subdivided according to the presence of crystals. Local cellular and humoral inflammatory mediators were analysed in the SF samples. The expression levels of IL-1β, IL-18, CASP-1, NLRP3, and GAPDH were measured by RT-PCR in the cells obtained by pelleting the SF samples. For the in vitro study, a monocytic cell line was treated with selected SF samples. SF with CPP crystals showed a significant increase in inflammatory cellular indices and higher levels of IL-1β, IL-8, and caspase-1 transcript with respect to SF without crystals. Higher concentrations of VEGF were also observed in the early stages of the whole OA patients. THP-1 cells stimulated with OA SF released a significant amount of IL-1 β in culture supernatants. This study demonstrated that SF collected from patients with OA shows different inflammatory features depending on the presence of CPP crystals.
焦磷酸钙（CPP）晶体在骨关节炎（OA）中的作用仍存在争议。本研究旨在探讨与无晶体 OA 患者相比，CPP 晶体 OA 患者滑液（SF）的炎症特征。我们还探讨了 OA SF 对单核细胞反应的影响。从 OA 成年患者中收集 SF，并根据晶体的存在进行细分。分析 SF 样本中的局部细胞和体液炎症介质。通过沉淀 SF 样本获得的细胞中，通过 RT-PCR 测量 IL-1β、IL-18、CASP-1、NLRP3 和 GAPDH 的表达水平。对于体外研究，用选定的 SF 样本处理单核细胞系。与无晶体 SF 相比，含有 CPP 晶体的 SF 显示出炎症细胞指数的显著增加，以及更高水平的 IL-1β、IL-8 和 caspase-1 转录物。在整个 OA 患者的早期阶段，还观察到更高浓度的 VEGF。用 OA SF 刺激 THP-1 细胞在培养上清液中释放出大量的 IL-1β。这项研究表明，根据 CPP 晶体的存在，OA 患者的 SF 表现出不同的炎症特征。
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