State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 311400, China.
College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
Int J Mol Sci. 2024 Jan 2;25(1):600. doi: 10.3390/ijms25010600.
False smut, caused by , is becoming increasingly serious in modern rice production systems, leading to yield losses and quality declines. Successful infection requires efficient acquisition of sucrose, abundant in rice panicles, as well as other sugars. Sugar transporters (STPs) may play an important role in this process. STPs belong to a major facilitator superfamily, which consists of large multigenic families necessary to partition sugars between fungal pathogens and their hosts. This study identified and characterized the STP family of , and further analyzed their gene functions to uncover their roles in interactions with rice. Through genome-wide and systematic bioinformatics analyses, 35 STPs were identified from V.virens and named from to . Transmembrane domains, gene structures, and conserved motifs of VvSTPs have been identified and characterized through the bioinformatic analysis. In addition, a phylogenetic analysis revealed relationship between VvSTPs and STPs from the other three reference fungi. According to a qRT-PCR and RNA-sequencing analysis, expression responded differently to different sole carbon sources and HO treatments, and changed during the pathogenic process, suggesting that these proteins are involved in interactions with rice and potentially functional in pathogenesis. In total, 12 representative VvSTPs were knocked out through genetic recombination in order to analyze their roles in pathogenicity of . The knock-out mutants of VvSTPs showed little difference in mycelia growth and conidiation, indicating a single gene in this family cannot influence vegetative growth of . It is clear, however, that these mutants result in a change in infection efficiency in a different way, indicating that VvSTPs play an important role in the pathogenicity of virens. This study is expected to contribute to a better understanding of how host-derived sugars contribute to pathogenicity.
假黑粉病，由 引起，在现代水稻生产系统中变得越来越严重，导致产量损失和质量下降。成功的感染需要有效地获取水稻穗部丰富的蔗糖以及其他糖分。糖转运蛋白（STPs）可能在这个过程中发挥重要作用。STPs 属于主要易化子超家族，该家族由真菌病原体与其宿主之间糖分分配所需的大型多基因家族组成。本研究鉴定并表征了 中的 STP 家族，并进一步分析了它们的基因功能，以揭示它们在与水稻相互作用中的作用。通过全基因组和系统生物信息学分析，从 V.virens 中鉴定并命名了 35 个 STP，从 到 。通过生物信息学分析鉴定和表征了 VvSTPs 的跨膜结构域、基因结构和保守基序。此外，系统发育分析揭示了 VvSTPs 与其他三种参考真菌的 STPs 之间的关系。根据 qRT-PCR 和 RNA-seq 分析，发现 对不同的单一碳源和 HO 处理的表达反应不同，并且在致病过程中发生变化，这表明这些蛋白参与了与水稻的相互作用，并可能在致病过程中具有功能。总共通过遗传重组敲除了 12 个代表性的 VvSTPs，以分析它们在 的致病性中的作用。VvSTPs 的敲除突变体在菌丝生长和产孢方面没有差异，这表明该家族中的单个基因不会影响 的营养生长。然而，很明显，这些突变体以不同的方式导致感染效率的变化，这表明 VvSTPs 在 的致病性中发挥重要作用。本研究有望有助于更好地理解宿主来源的糖如何有助于 的致病性。
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