Feng Kai, Yang Zhi-Yuan, Yan Ya-Jie, Sun Nan, Zhou Zi-Qi, Liu Jia-Lu, Zhao Shu-Ping, Wu Peng, Li Liang-Jun
College of Horticulture and Landscape Architecture, Yangzhou University, Yangzhou, China.
Joint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of China, Yangzhou University, Yangzhou, China.
Front Plant Sci. 2023 Dec 27;14:1287589. doi: 10.3389/fpls.2023.1287589. eCollection 2023.
Gene expression analysis is widely used to unravel molecular regulatory mechanisms and identify key genes in plants. Appropriate reference gene is an important prerequisite to ensure the accuracy and reliability of qPCR analysis results. Water dropwort is a plant of the Oenanthe genus in the Apiaceae family, which has high economic benefits. However, the underlying molecular regulatory mechanisms in the growth and development of water dropwort have not been fully understood and the appropriate reference genes in different developmental stages of water dropwort not yet reported. In this study, 10 candidate reference genes () were identified and cloned from . The qPCR primers of candidate reference genes were designed and verified. Four statistical algorithms, geNorm, NormFinder, BestKeeper and RefFinder were used to evaluate the expression stability of 10 candidate reference genes in different developmental stages of water dropwort. The results showed that and were the most stable genes in different developmental stages of water dropwort, while was the most unstable gene. The normalization of genes at different developmental stages further confirmed the reliability of internal reference genes. The results of this study provide a theoretical basis for selecting appropriate internal reference genes in different developmental stages of water dropwort. This study also provides technical support and reliable basis for the expression analysis of key genes in different developmental stages of water dropwort.
基因表达分析被广泛用于揭示植物中的分子调控机制并鉴定关键基因。合适的内参基因是确保qPCR分析结果准确性和可靠性的重要前提。水芹是伞形科水芹属的一种植物，具有很高的经济效益。然而，水芹生长发育的潜在分子调控机制尚未完全明确，且水芹不同发育阶段合适的内参基因尚未见报道。在本研究中，从[具体来源未给出]中鉴定并克隆了10个候选内参基因（[此处基因名称未给出]）。设计并验证了候选内参基因的qPCR引物。使用geNorm、NormFinder、BestKeeper和RefFinder四种统计算法评估这10个候选内参基因在水芹不同发育阶段的表达稳定性。结果表明，[此处基因名称未给出]和[此处基因名称未给出]是水芹不同发育阶段最稳定的基因，而[此处基因名称未给出]是最不稳定的基因。对不同发育阶段[此处基因数量未给出]个基因的标准化进一步证实了内参基因的可靠性。本研究结果为水芹不同发育阶段选择合适的内参基因提供了理论依据。本研究也为水芹不同发育阶段关键基因的表达分析提供了技术支持和可靠依据。
