Vu Mai Phuong, Le Hanh Tran Nguyen, Lam Thien Quang, Quynh Tran Anh Thi, Anh Le Thu Phan, Nguyen Khoi Tan
School of Biotechnology, International University, Vietnam National University Ho Chi Minh City 700000 Vietnam
RSC Adv. 2024 Jan 10;14(3):2159-2169. doi: 10.1039/d3ra07454d. eCollection 2024 Jan 3.
Several previous studies have considered ultrafine bubbles as a potential research target because their properties can be applied in many different research areas. In particular, the interaction between UFBs and microorganisms has always been one of the aspects that receives much attention due to the high difficulty in controlling a living system. The properties of UFBs, as mobile air-water interfaces, are greatly determined by their gas cores which play a critical role in regulating microbial growth. This study aims to investigate the effects of ultrafine bubbles on bacterial growth. Two well-studied organisms were chosen as models - and . Their growing behavior was examined based on the growth rate, phenotype and biomass. Three types of Luria-Bertani cultures were tested, including a standard culture containing distilled water, an air ultrafine bubble culture, and a hydrogen ultrafine bubble culture. The UFBs were generated ultrasonic cavitation and stabilized by 50 μM SDS, which was proven to have negligible effects on bacterial growth. By comparing among the three cultivation conditions, the bacterial growth rates were observed to be the highest in exposure to HUFBs. The results also signified that UFBs had an enhancement on cell proliferation. On the other hand, while proposing an increase in cell density, bacteria cultured in HUFB media have their sizes decreased uniformly and significantly (-value < 0.05). This study confirmed that bacterial growth was promoted by UFBs; and better effects recorded were due to the HUFB present in the culture media. However, the average morphological size of bacteria was in negative correlation with their population size.
先前的几项研究已将超细气泡视为一个潜在的研究目标，因为其特性可应用于许多不同的研究领域。特别是，由于控制生物系统的难度很大，超细气泡与微生物之间的相互作用一直是备受关注的方面之一。作为移动的气 - 水界面，超细气泡的特性很大程度上由其气核决定，气核在调节微生物生长中起着关键作用。本研究旨在探究超细气泡对细菌生长的影响。选择了两种经过充分研究的生物体作为模型—— 和 。基于生长速率、表型和生物量来检查它们的生长行为。测试了三种类型的Luria - Bertani培养基，包括含有蒸馏水的标准培养基、空气超细气泡培养基和氢气超细气泡培养基。通过超声空化产生超细气泡，并用50μM十二烷基硫酸钠（SDS）使其稳定，已证明该物质对细菌生长的影响可忽略不计。通过比较三种培养条件，观察到在暴露于氢气超细气泡时细菌生长速率最高。结果还表明超细气泡对细胞增殖有促进作用。另一方面，虽然在氢气超细气泡培养基中培养的细菌细胞密度增加，但其大小均匀且显著减小（-值<0.05）。本研究证实超细气泡促进了细菌生长；记录到的更好效果归因于培养基中存在的氢气超细气泡。然而，细菌的平均形态大小与其种群大小呈负相关。
