Robin O, Berard M
Pathol Biol (Paris). 1979 Mar;27(3):178-80.
A simple method for the assay of post-heparin plasma lipoprotein lipase activity is described. The substrate was a stable intralipid solution. The amount of fatty acids released in the 37 degrees C incubation medium was measured several time during the reaction course, by light absorption on a Technicon auto-analyzer. By plotting these values on a graph paper, the curve of the post-heparin plasma lipoprotein lipase activity was obtained.
本文描述了一种检测肝素后血浆脂蛋白脂肪酶活性的简单方法。底物是一种稳定的脂肪乳剂溶液。在反应过程中,通过Technicon自动分析仪上的光吸收,在37℃孵育培养基中多次测量释放的脂肪酸量。将这些值绘制在坐标纸上,即可得到肝素后血浆脂蛋白脂肪酶活性曲线。