Yoshimura H, Takemoto K, Fukuda K, Matsushita H
Sangyo Igaku. 1986 Sep;28(5):352-9. doi: 10.1539/joh1959.28.352.
Benzotrichloride and benzoyl chloride are suspected to be causative agents of lung cancer and maxillary malignant lymphoma of workers employed in factories producing benzoyl chloride. The present study was undertaken to evaluate the carcinogenicity in mice of inhaling benzotrichloride and benzoyl chloride. Mice inhaled benzotrichloride and benzoyl chloride vapor for 30 min/d for 2 d/wk for 5 months, and each animal was followed for several month without subsequent exposure. Tumor developed in the lung, skin and lymphatic tissues at high incidences in mice inhaling benzotrichloride. By benzotrichloride vaporized at 50 degrees C, the incidence of pulmonary tumors was 53.1% (17/32, p less than 0.001), that of skin tumors was 25% (8/32, p less than 0.02), and that of malignant lymphoma was 25% (8/32, p less than 0.02) observed at the 10th month after exposure. These are significantly higher than that observed in control mice. In mice exposed to benzotrichloride vaporized at room temperature, the incidence of pulmonary tumors was 81.1% (30/37), that of skin tumors was 27.0% (10/37), and that of malignant lymphoma was 10.8% (4/37) observed at the 15th month after exposure. On the other hand, by benzoyl chloride vaporized at 50 degrees C, the incidence of pulmonary tumors was 10.7% (3/28) and that of skin tumors was 7.1% (2/28), but these incidences did not show any significant difference from the controls. These results suggest that the carcinogenicity of benzotrichloride is much higher than benzoyl chloride and that benzotrichloride is the primary cause of malignancies developing among workers engaged in manufacturing benzoyl chloride.
三氯甲苯和苯甲酰氯被怀疑是生产苯甲酰氯工厂工人肺癌和上颌恶性淋巴瘤的致病因素。本研究旨在评估吸入三氯甲苯和苯甲酰氯对小鼠的致癌性。小鼠每周吸入三氯甲苯和苯甲酰氯蒸气2天，每天30分钟，持续5个月，然后对每只动物进行数月的观察，不再进行后续暴露。吸入三氯甲苯的小鼠肺部、皮肤和淋巴组织中肿瘤的发生率很高。在50℃蒸发的三氯甲苯作用下，暴露后第10个月观察到肺部肿瘤发生率为53.1%（17/32，p<0.001），皮肤肿瘤发生率为25%（8/32，p<0.02），恶性淋巴瘤发生率为25%（8/32，p<0.02）。这些发生率显著高于对照组小鼠。在暴露于室温下蒸发的三氯甲苯的小鼠中，暴露后第15个月观察到肺部肿瘤发生率为81.1%（30/37），皮肤肿瘤发生率为27.0%（10/37），恶性淋巴瘤发生率为10.8%（4/37）。另一方面，在50℃蒸发的苯甲酰氯作用下，肺部肿瘤发生率为10.7%（3/28），皮肤肿瘤发生率为7.1%（2/28），但这些发生率与对照组相比没有显著差异。这些结果表明，三氯甲苯的致癌性远高于苯甲酰氯，且三氯甲苯是从事苯甲酰氯生产的工人发生恶性肿瘤的主要原因。
