Gomez-Sanchez C E, Ferris M W, Foecking M F, Gomez-Sanchez E P
Department of Internal Medicine, University of South Florida College of Medicine, Tampa.
J Steroid Biochem. 1989 Oct;33(4A):595-8. doi: 10.1016/0022-4731(89)90046-0.
The adrenal cortex shows a histological and functional zonation which allows it to function as two distinct glands. Aldosterone is produced only in the outer area, or zona glomerulosa and cortisol is produced in the zona fasciculata. Adrenal slices (200 mu) were prepared from cores of beef adrenals using a McIlwain Tissue Slicer and incubated. Cortisol, corticosterone, aldosterone, 18-hydroxycorticosterone, 18-hydroxycortisol and 18-oxocortisol were measured by radioimmunoassay. Aldosterone and 18-hydroxycorticosterone were produced primarily in the outer slices of the adrenal. Cortisol and corticosterone were produced throughout the adrenal with the production of cortisol in the first layer probably due to contamination with fasciculata. 18-Hydroxycortisol and 18-oxocortisol were produced primarily in the outer slices. The coexistence of cytochrome P-450 corticosterone methyl oxidase I and II and the 17 alpha-hydroxylase in the outer slices leads to the synthesis of these two hybrid steroids.
肾上腺皮质呈现出组织学和功能分区，使其能够像两个不同的腺体一样发挥作用。醛固酮仅在外部区域即球状带产生，而皮质醇则在束状带产生。使用麦基尔温组织切片机从牛肉肾上腺的核心制备肾上腺切片（200微米）并进行孵育。通过放射免疫测定法测量皮质醇、皮质酮、醛固酮、18 - 羟皮质酮、18 - 羟皮质醇和18 - 氧皮质醇。醛固酮和18 - 羟皮质酮主要在肾上腺的外层切片中产生。皮质醇和皮质酮在整个肾上腺中产生，第一层中皮质醇的产生可能是由于受到束状带的污染。18 - 羟皮质醇和18 - 氧皮质醇主要在外部切片中产生。外层切片中细胞色素P - 450皮质酮甲基氧化酶I和II与17α - 羟化酶的共存导致了这两种杂合类固醇的合成。
