Nucleus of Analysis and Research in Nuclear Magnetic Resonance - NAPRMN, Institute of Chemistry and Biotechnology, Federal University of Alagoas, Maceió, AL, Brazil.
Institute of Biological and Health Sciences, Federal University of Alagoas, Maceió, AL, Brazil.
Environ Toxicol Pharmacol. 2024 Mar;106:104361. doi: 10.1016/j.etap.2024.104361. Epub 2024 Jan 9.
Thimerosal, a preservative commonly used in the pharmaceutical and cosmetic industry, has raised concerns regarding its potentially toxic effects as an organic mercury compound. Within this context, using an NMR-based metabolomics profile and chemometric analysis, zebrafish embryos were used as an in vivo model to study the effects of thimerosal in metabolic profiles after exposure to sublethal concentrations of the mercury compound. The thimerosal concentrations of 40 and 80 nM were employed, corresponding to 40% and 80% of the LC, respectively, for zebrafish embryos. The most significant alterations in the metabolic profile included changes in carbohydrates, amino acids, nucleotides, trimethylamine-N-oxide, ethanolamine, betaine, and ethanol. Furthermore, thimerosal exposure affects various metabolic pathways, impairing the nervous system, disrupting protein metabolism, and potentially causing oxidative damage. Therefore, adopting a metabolomics approach in this investigation provided insights into the potentially implicated metabolic pathways contributing to the deleterious effects of thimerosal in biological systems.
硫柳汞是一种在制药和化妆品行业中常用的防腐剂，因其作为有机汞化合物具有潜在的毒性作用而引起关注。在此背景下，本研究采用基于 NMR 的代谢组学分析和化学计量学分析方法，以斑马鱼胚胎为体内模型，研究了亚致死浓度的汞化合物暴露后，硫柳汞对代谢谱的影响。实验采用的硫柳汞浓度分别为 40 和 80 nM，分别相当于斑马鱼胚胎 LC50 的 40%和 80%。代谢谱的最显著变化包括碳水化合物、氨基酸、核苷酸、三甲胺 N-氧化物、乙醇胺、甜菜碱和乙醇的变化。此外，硫柳汞暴露还会影响多种代谢途径，损害神经系统，扰乱蛋白质代谢，并可能导致氧化损伤。因此，本研究采用代谢组学方法，深入探讨了硫柳汞在生物系统中产生有害影响所涉及的潜在代谢途径。
