Murthy V K, Oredipe O A, Stiles M R, Shipp J C
Mech Ageing Dev. 1986 Nov 28;37(1):49-54. doi: 10.1016/0047-6374(86)90117-x.
The mechanisms responsible for the increased hepatic triacylglycerol synthesis in aging are not established. We studied [1-14C] palmitate uptake and its esterification to triacylglycerols in the isolated hepatocytes of 2-month, 10-month and 20-month-old normal rats. In all hepatocytes, palmitate uptake and its esterification were linearly related to medium palmitate concentration, but palmitate uptake and triacylglycerol synthesis by the hepatocytes of 10-month and 20-month-old rats were nearly double that observed with the cells of 2-month-old rats. These results suggest that increased fatty acid uptake by the liver cells was a contributory factor in the increased triacylglycerol synthesis observed in the liver of senescent rats. The changes in the hepatocyte leading to increased fatty acid uptake and hence increased triacylglycerol synthesis are detected as early as middle age of the rat.
衰老过程中肝脏三酰甘油合成增加的机制尚未明确。我们研究了2月龄、10月龄和20月龄正常大鼠分离肝细胞中[1-14C]棕榈酸的摄取及其酯化生成三酰甘油的情况。在所有肝细胞中，棕榈酸摄取及其酯化与培养基中棕榈酸浓度呈线性相关，但10月龄和20月龄大鼠肝细胞的棕榈酸摄取和三酰甘油合成几乎是2月龄大鼠细胞的两倍。这些结果表明，肝细胞脂肪酸摄取增加是衰老大鼠肝脏中观察到的三酰甘油合成增加的一个促成因素。导致脂肪酸摄取增加进而三酰甘油合成增加的肝细胞变化早在大鼠中年时就已被检测到。
