膜技术在 RNA 和 DNA 治疗药物纯化中的应用。

Membrane technology for the purification of RNA and DNA therapeutics.

机构信息

Department of Chemical Engineering, The Pennsylvania State University, University Park, PA 16802, USA.

出版信息

Trends Biotechnol. 2024 Jun;42(6):714-727. doi: 10.1016/j.tibtech.2023.11.016. Epub 2024 Jan 11.

DOI:10.1016/j.tibtech.2023.11.016

Abstract

Nucleic acid therapeutics have the potential to revolutionize the biopharmaceutical industry, providing highly effective vaccines and novel treatments for cancers and genetic disorders. The successful commercialization of these therapeutics will require development of manufacturing strategies specifically tailored to the purification of nucleic acids. Membrane technologies already play a critical role in the downstream processing of nucleic acid therapeutics, ranging from clarification to concentration to selective purification. This review provides an overview of how membrane systems are currently used for nucleic acid purification, while highlighting areas of future need and opportunity, including adoption of membranes in continuous bioprocessing.

摘要

核酸疗法有可能彻底改变生物制药行业，为癌症和遗传疾病提供高效疫苗和新型治疗方法。这些疗法的成功商业化将需要开发专门针对核酸纯化的制造策略。膜技术已经在核酸疗法的下游处理中发挥着至关重要的作用，从澄清到浓缩再到选择性纯化。本文综述了膜系统目前如何用于核酸纯化，同时强调了未来的需求和机会领域，包括在连续生物加工中采用膜。