伊朗霍拉马巴德地区耐多药和广泛耐药分离株中、基因的研究。

Investigation of , and Genes among MDR and XDR Isolates in Khorramabad, Iran.

机构信息

School of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran.

Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran.

出版信息

Recent Adv Antiinfect Drug Discov. 2024;19(4):307-314. doi: 10.2174/0127724344274260231220052526.

DOI:10.2174/0127724344274260231220052526

PMID:38213149

Abstract

BACKGROUND

is an opportunistic hospital pathogen with high antibiotic resistance, and the ability to produce biofilm. This study aimed to investigate epsA, ompA, and bap genes involved in biofilm formation in MDR and XDR clinical isolates of in Khorramabad, Iran.

METHODS

In this study, 79 isolates were collected from various samples of the patients admitted to tertiary hospitals in Khorramabad city, Iran, between January and August 2019. After performing the semi-quantitative evaluation of biofilm production by microtiter plate assay, screening of isolates carrying , and genes was done by PCR method. Finally, statistical analyses were conducted using SPSS 22.

RESULTS

Among 79 isolates, 52% XDR, 40% MDR, and 16% non-XDRMDR isolates were found to be biofilm producers. All XDR and 94% MDR isolates had and genes, and genes were detected among > 80% of these isolates. Moreover, the presence of biofilm-related genes and biofilm production among non-XDRMDR isolates were less than among resistant isolates (p≤ 0.01).

CONCLUSION

Based on the results, biofilm production and simultaneous presence of epsA, ompA, and bap genes among MDR, and XDR isolates have been found to be significantly more than non-XDR-MDR isolates.

摘要

背景

是一种机会性病原体，具有很高的抗生素耐药性和形成生物膜的能力。本研究旨在研究生物膜形成中涉及的 epsA、ompA 和 bap 基因在伊朗霍拉马巴德的 MDR 和 XDR 临床分离株中。

方法

本研究中，从 2019 年 1 月至 8 月期间伊朗霍拉马巴德市三级医院的患者各种样本中收集了 79 株分离株。通过微量滴定板测定法进行生物膜产生的半定量评价后，通过 PCR 法筛选携带、和基因的分离株。最后使用 SPSS 22 进行统计分析。

结果

在 79 株分离株中，发现 52%的 XDR、40%的 MDR 和 16%的非 XDR-MDR 分离株为生物膜生产者。所有 XDR 和 94%的 MDR 分离株均具有和基因，并且这些分离株中的> 80%都检测到了基因。此外，非 XDR-MDR 分离株中生物膜相关基因的存在和生物膜产生的情况均少于耐药分离株（p≤0.01）。