Clin Lab. 2024 Jan 1;70(1). doi: 10.7754/Clin.Lab.2023.230434.
The goal was to assess the antimicrobial efficacy of two commonly used biocides, chlorhexidine, and benzalkonium chloride, against MDR isolates of Pseudomonas aeruginosa, Acinetobacter baumannii, and Escherichia coli ST131, as well as the prevalence of resistance genes.
MIC of chlorhexidine and benzalkonium chloride and their effects on both the planktonic phase and biofilm were determined. Finally, the presence of genes responsible for resistance to quaternary ammonium compounds was investigated by PCR.
No significant relationship was observed between the presence of resistance genes and different concentrations of quaternary ammonium compounds (benzalkonium chloride). There was no association between biofilm formation and the presence of resistance genes.
Chlorhexidine digluconate and benzalkonium chloride at appropriate concentrations could prevent biofilm formation.
本研究旨在评估两种常用消毒剂——洗必泰（chlorhexidine）和苯扎氯铵（benzalkonium chloride）对多重耐药的铜绿假单胞菌、鲍曼不动杆菌和产 ESBLs 大肠杆菌 ST131 的抗菌效果，并检测其耐药基因的流行情况。
测定洗必泰和苯扎氯铵的 MIC 值，观察其对浮游期和生物膜的影响，并通过 PCR 检测负责抵抗季铵化合物的基因的存在。
未发现耐药基因的存在与不同浓度的季铵化合物（苯扎氯铵）之间存在显著关系。生物膜形成与耐药基因的存在之间也没有关联。
洗必泰葡萄糖酸盐和苯扎氯铵在适当的浓度下可以预防生物膜的形成。
