Kumar V V, Raghunathan P
Lipids. 1986 Dec;21(12):764-8. doi: 10.1007/BF02535409.
The nature of the water pool formed in the reverse micellar system, lecithin/nonpolar solvent/water, has been investigated by means of near infrared, ultraviolet, fluorescence emission and visible spectroscopic techniques. The three nonpolar solvents chosen in this study were benzene, carbon tetrachloride and cyclohexane. Near infrared spectroscopic studies revealed that the amount of water present in the bulk organic phase is negligible at all water concentrations studied in all three solvents. The results of the polarity probe and 8-anilinonaphthalene sulfonic acid (ANSA) fluorescence emission maxima studies indicate that the polarity of the water pool is much lower than that of bulk water. The difference in polarity between the water pool and bulk water decreases with increasing water concentration in benzene and carbon tetrachloride systems. However, in the cyclohexane system, at a water content of 6 moles of water per mole of lecithin, where the system is known to change from isotropic reverse micelle to anisotropic liquid crystalline state, the polarity of the water pool is found to decrease.
已通过近红外、紫外、荧光发射和可见光谱技术研究了在反胶束体系卵磷脂/非极性溶剂/水中形成的水池的性质。本研究中选择的三种非极性溶剂为苯、四氯化碳和环己烷。近红外光谱研究表明，在所有三种溶剂中所研究的所有水浓度下，本体有机相中存在的水量均可忽略不计。极性探针和8-苯胺基萘磺酸（ANSA）荧光发射最大值研究的结果表明，水池的极性远低于本体水的极性。在苯和四氯化碳体系中，随着水浓度的增加，水池与本体水之间的极性差异减小。然而，在环己烷体系中，当水含量为每摩尔卵磷脂6摩尔水时，已知该体系从各向同性反胶束转变为各向异性液晶态，此时发现水池的极性降低。
