Leser M E, Wei G, Luisi P L, Maestro M
Biochem Biophys Res Commun. 1986 Mar 13;135(2):629-35. doi: 10.1016/0006-291x(86)90039-2.
The solubilization of proteins in hydrocarbon solutions of reverse micelles is examined at the aim of establishing whether this process can be utilized for the separation and extraction of proteins. Two techniques of solubilization are considered, the phase transfer of proteins from an aqueous solution into a supernatant micellar solution and the direct extraction of the protein powder into the micellar solution. Basic questions concerning the influence of structural parameters of the proteins, as well as the influence of external parameters (pH, salt concentration) on the specificity of the solubilization process are discussed.
研究了蛋白质在反胶束碳氢化合物溶液中的增溶作用，目的是确定该过程是否可用于蛋白质的分离和提取。考虑了两种增溶技术，即蛋白质从水溶液到上清胶束溶液的相转移以及蛋白质粉末直接提取到胶束溶液中。讨论了有关蛋白质结构参数的影响以及外部参数（pH值、盐浓度）对增溶过程特异性影响的基本问题。
