Department of Chemical Engineering, Osaka Metropolitan University, 1-1 Gakuen-Cho, Naka-Ku, Sakai, Osaka, 599-8531, Japan.
Arch Microbiol. 2024 Jan 12;206(2):61. doi: 10.1007/s00203-023-03796-2.
It is known that co-cultivation of green algae with heterotrophic microorganisms, such as yeast, improves green algae's growth potential and carbon dioxide fixation, even under low CO concentration conditions such as the atmosphere. Introducing mutations into green algae is also expected to enhance their growth potential. In this study, we sought to improve the growth potential of a co-culture system of the green algae Chlamydomonas reinhardtii and the yeast Saccharomyces cerevisiae by introducing mutations into the green algae. Additionally, we performed a transcriptome analysis of the co-culture of the green algae mutant strain with yeast, discussing the interaction between the green algae mutant strain and the yeast. When the green algae mutant strain was co-cultured with yeast, the number of green algae cells reached 152 × 10 cells/mL after 7 days of culture. This count was 2.6 times higher than when the wild-type green algae strain was cultured alone and 1.6 times higher than when the wild-type green algae strain and yeast were co-cultured. The transcriptome analysis also indicated that the primary reason for the increased growth potential of the green algae mutant strain was its enhanced photosynthetic activity and nitrogen utilization efficiency.
已知在低 CO 浓度条件下（如大气中），与异养微生物（如酵母）共培养绿藻可以提高绿藻的生长潜力和二氧化碳固定能力。引入突变也有望增强绿藻的生长潜力。在这项研究中，我们试图通过向绿藻莱茵衣藻中引入突变来提高绿藻和酵母酿酒酵母的共培养系统的生长潜力。此外，我们对绿藻突变株与酵母的共培养进行了转录组分析，讨论了绿藻突变株与酵母之间的相互作用。当绿藻突变株与酵母共培养时，培养 7 天后绿藻细胞数达到 152×10^6 个/mL，是单独培养野生型绿藻时的 2.6 倍，是野生型绿藻和酵母共培养时的 1.6 倍。转录组分析还表明，绿藻突变株生长潜力增加的主要原因是其增强的光合作用活性和氮利用效率。
