莱茵衣藻两个新的紫外线敏感突变体的一般特征、分子和遗传分析

General characteristics, molecular and genetic analysis of two new UV-sensitive mutants of Chlamydomonas reinhardtii.

作者信息

Vlcek D, Podstavkova S, Miadokova E, Adams G M, Small G D

出版信息

Mutat Res. 1987 Mar;183(2):169-75. doi: 10.1016/0167-8817(87)90059-9.

Abstract

Two new UV-sensitive mutants of Chlamydomonas, UVS10 and UVS11, were isolated. Both behave as single nuclear mutations. UVS10 was mapped to linkage group I. UVS11 is a separate, unlinked mutation but has not yet been located to a specific linkage group. Both mutants are proficient in the excision of pyrimidine dimers from nuclear DNA. The survival of UV-irradiated UVS11 is increased when plated in the presence of 1.5 mM caffeine, similar to wild-type. Caffeine has no effect on the survival of UV-irradiated UVS10. UV-irradiated UVS11 frequently divides at least once before dying, in contrast to UVS10 or wild-type. UVS11 also exhibits a much increased frequency of mutation to streptomycin resistance after UV irradiation.

摘要

分离出了衣藻的两个新的紫外线敏感突变体UVS10和UVS11。二者均表现为单核基因突变。UVS10被定位到连锁群I。UVS11是一个单独的、不连锁的突变体，但尚未定位到特定的连锁群。两个突变体都能有效地从核DNA中切除嘧啶二聚体。当在1.5 mM咖啡因存在的情况下平板接种时，紫外线照射后的UVS11的存活率增加，与野生型相似。咖啡因对紫外线照射后的UVS10的存活率没有影响。与UVS10或野生型相比，紫外线照射后的UVS11在死亡前经常至少分裂一次。UVS11在紫外线照射后对链霉素抗性的突变频率也显著增加。